Bone-Conditioned Medium Changes Gene Expression in Bone-Derived Fibroblasts.

Zimmermann, Matthias; Caballé Serrano, Jordi; Bosshardt, Dieter; Ankersmit, Hendrik J; Buser, Daniel; Gruber, Reinhard (2015). Bone-Conditioned Medium Changes Gene Expression in Bone-Derived Fibroblasts. The international journal of oral & maxillofacial implants, 30(4), pp. 953-958. Quintessence Publishing 10.11607/jomi.4060

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PURPOSE Autologous bone is used for augmentation in the course of oral implant placement. Bone grafts release paracrine signals that can modulate mesenchymal cell differentiation in vitro. The detailed genetic response of the bone-derived fibroblasts to these paracrine signals has remained elusive. Paracrine signals accumulate in bone-conditioned medium (BCM) prepared from porcine cortical bone chips. MATERIALS AND METHODS In this study, bone-derived fibroblasts were exposed to BCM followed by a whole genome expression profiling and downstream quantitative reverse transciptase polymerase chain reaction of the most strongly regulated genes. RESULTS The data show that ADM, IL11, IL33, NOX4, PRG4, and PTX3 were differentially expressed in response to BCM in bone-derived fibroblasts. The transforming growth factor beta (TGF-β) receptor 1 antagonist SB431542 blocked the effect of BCM on the expression of the gene panel, except for IL33. CONCLUSION These in vitro results extend existing evidence that cortical bone chips release paracrine signals that provoke a robust genetic response in mesenchymal cells that is not exclusively mediated via the TGF-β receptor. The present data provide further insights into the process of graft consolidation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology

UniBE Contributor:

Zimmermann, Matthias; Caballé Serrano, Jordi; Bosshardt, Dieter; Buser, Daniel and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1942-4434

Publisher:

Quintessence Publishing

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

19 Oct 2015 10:27

Last Modified:

31 Jul 2019 09:05

Publisher DOI:

10.11607/jomi.4060

PubMed ID:

26252049

Uncontrolled Keywords:

ADM, bone-conditioned medium, bone supernatant, IL11, IL33, NOX4, PRG4, PTX3, SB431542

URI:

https://boris.unibe.ch/id/eprint/72293

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