Pre-clinical in vivo models for the screening of bone biomaterials for oral/craniofacial indications: focus on small-animal models.

Stavropoulos, Andreas; Sculean, Anton; Bosshardt, Dieter; Buser, Daniel; Klinge, Björn (2015). Pre-clinical in vivo models for the screening of bone biomaterials for oral/craniofacial indications: focus on small-animal models. Periodontology 2000, 68(1), pp. 55-65. Blackwell 10.1111/prd.12065

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Preclinical in vivo experimental studies are performed for evaluating proof-of-principle concepts, safety and possible unwanted reactions of candidate bone biomaterials before proceeding to clinical testing. Specifically, models involving small animals have been developed for screening bone biomaterials for their potential to enhance bone formation. No single model can completely recreate the anatomic, physiologic, biomechanic and functional environment of the human mouth and jaws. Relevant aspects regarding physiology, anatomy, dimensions and handling are discussed in this paper to elucidate the advantages and disadvantages of small-animal models. Model selection should be based not on the 'expertise' or capacities of the team, but rather on a scientifically solid rationale, and the animal model selected should reflect the question for which an answer is sought. The rationale for using heterotopic or orthotopic testing sites, and intraosseous, periosseous or extraskeletal defect models, is discussed. The paper also discusses the relevance of critical size defect modeling, with focus on calvarial defects in rodents. In addition, the rabbit sinus model and the capsule model in the rat mandible are presented and discussed in detail. All animal experiments should be designed with care and include sample-size and study-power calculations, thus allowing generation of meaningful data. Moreover, animal experiments are subject to ethical approval by the relevant authority. All procedures and the postoperative handling and care, including postoperative analgesics, should follow best practice.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine

UniBE Contributor:

Stavropoulos, Andreas; Sculean, Anton; Bosshardt, Dieter and Buser, Daniel

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0906-6713

Publisher:

Blackwell

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

19 Oct 2015 13:24

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1111/prd.12065

PubMed ID:

25867979

BORIS DOI:

10.7892/boris.72296

URI:

https://boris.unibe.ch/id/eprint/72296

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