Osteoclast-like cells on deproteinized bovine bone mineral and biphasic calcium phosphate: light and transmission electron microscopical observations.

Jensen, Simon Storgaard; Gruber, Reinhard; Buser, Daniel; Bosshardt, Dieter (2015). Osteoclast-like cells on deproteinized bovine bone mineral and biphasic calcium phosphate: light and transmission electron microscopical observations. Clinical oral implants research, 26(8), pp. 859-864. Wiley-Blackwell 10.1111/clr.12376

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OBJECTIVES The occurrence of multinucleated giant cells (MNGCs) on bone substitute materials has been recognized for a long time. However, there have been no studies linking material characteristics with morphology of the MNGCs. The aim was to analyze the qualitative differences of MNGCs on two commercially available calcium phosphate bone substitute materials retrieved from bone defects. MATERIAL AND METHODS Six defects were prepared bilaterally in the mandibular body of three mini pigs. The defects were randomly grafted with either deproteinized bovine bone mineral (DBBM) or biphasic calcium phosphate (BCP). After a healing period of four weeks, bone blocks were embedded in LR White resin. Three consecutive sections per defect were analyzed as follows: two with light microscopy using toluidine blue and tartrate-resistant acid phosphatase (TRAP) staining and one with transmission electron microscopy. RESULTS Multinucleated giant cells appeared on both biomaterials. On BCP, MNGCs had a flat morphology and were not observed in resorption lacunae. On DBBM, the MNGCs appeared more round and were often found in shallow concavities. MNGCs on both biomaterials demonstrated a varying degree of TRAP staining, with a tendency toward higher staining intensity of MNGCs on BCP. At the ultrastructural level, signs of superficial dissolution of BCP together with phagocytosis of minor fragments were observed. MNGCs on the surface of DBBM demonstrated sealing zones and ruffled borders, both features of mature osteoclasts. CONCLUSION MNGCs demonstrated distinctly different histological features depending on the bone substitute material used. Further research is warranted to understand the clinical implications of these morphological observations.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology

UniBE Contributor:

Jensen, Simon Storgaard; Gruber, Reinhard; Buser, Daniel and Bosshardt, Dieter

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0905-7161

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

29 Oct 2015 10:50

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1111/clr.12376

PubMed ID:

24665833

Uncontrolled Keywords:

biphasic calcium phosphate, bone regeneration, degradation, deproteinized bovine bone mineral, osteoclast

BORIS DOI:

10.7892/boris.72318

URI:

https://boris.unibe.ch/id/eprint/72318

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