Salivary Pellicle vs Whole Saliva: The Response of Oral Fibroblasts Based on a Genome-Wide Microarray.

Cvikl, Barbara; Lussi, Adrian; Moritz, Andreas; Gruber, Reinhard (2015). Salivary Pellicle vs Whole Saliva: The Response of Oral Fibroblasts Based on a Genome-Wide Microarray. The international journal of oral & maxillofacial implants, 30(3), pp. 569-577. Quintessence Publishing 10.11607/jomi.3912

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Whole saliva comprises components of the salivary pellicle that spontaneously forms on surfaces of implants and teeth. However, there are no studies that functionally link the salivary pellicle with a possible change in gene expression.


This study examined the genetic response of oral fibroblasts exposed to the salivary pellicle and whole saliva. Oral fibroblasts were seeded onto a salivary pellicle and the respective untreated surface. Oral fibroblasts were also exposed to freshly harvested sterile-filtered whole saliva. A genome-wide microarray of oral fibroblasts was performed, followed by gene ontology screening with DAVID functional annotation clustering, KEGG pathway analysis, and the STRING functional protein association network.


Exposure of oral fibroblasts to saliva caused 61 genes to be differentially expressed (P < .05). Gene ontology screening assigned the respective genes into 262 biologic processes, 3 cellular components, 13 molecular functions, and 7 pathways. Most remarkable was the enrichment in the inflammatory response. None of the genes regulated by whole saliva was significantly changed when cells were placed onto a salivary pellicle.


The salivary pellicle per se does not provoke a significant inflammatory response of oral fibroblasts in vitro, whereas sterile-filtered whole saliva does produce a strong inflammatory response.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Restorative Dentistry, Research
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Cvikl, Barbara, Lussi, Adrian, Gruber, Reinhard


600 Technology > 610 Medicine & health




Quintessence Publishing




Eveline Carmen Schuler

Date Deposited:

30 Oct 2015 11:19

Last Modified:

05 Dec 2022 14:49

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

cytokines, fibroblasts, inflammation, microarray, saliva, salivary pellicle


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