The in vitro impact of toothpaste extracts on cell viability.

Cvikl, Barbara; Lussi, Adrian; Gruber, Reinhard (2015). The in vitro impact of toothpaste extracts on cell viability. European journal of oral sciences, 123(3), pp. 179-185. Wiley-Blackwell 10.1111/eos.12177

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Toothpastes contain three main components: detergents, abrasives, and fluoride. Detergents, particularly sodium lauryl sulfate, have been proposed as components that enable toothpastes to produce cytotoxic effects in vitro. However, not all toothpastes contain sodium lauryl sulfate, and almost no studies have found an association between detergents and the in vitro cytotoxicity of toothpastes. The present study examined the in vitro cytotoxicity of nine commercially available toothpastes containing four different detergents. Toothpastes were diluted in serum-free medium, centrifuged, and filter sterilized. The half-lethal concentration of the toothpaste-conditioned medium (TCM) was calculated based on the formation of formazan by gingival fibroblasts, oral squamous cell carcinoma HSC-2 cells, and L929 cells. Cell proliferation was analyzed, and live-dead staining was performed, after exposure of cells to conditioned medium prepared with 1% toothpaste (1% TCM). It was found that toothpastes containing sodium lauryl sulfate and amine fluoride strongly inhibited cell viability with the half-lethal concentration being obtained with conditioned medium prepared with approximately 1% toothpaste (1% TCM). Toothpastes containing cocamidopropyl betaine and Steareth-20 showed higher half-lethal concentration values, with the half-lethal concentration being obtained with conditioned medium prepared with 10% (10% TCM) and 70% (70% TCM) toothpaste, respectively. Proliferation and live-dead data were consistent with the cell-viability analyses. These results demonstrate that the type of detergent in toothpastes can be associated with changes in in vitro cell toxicity.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Restorative Dentistry, Research
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Cvikl, Barbara; Lussi, Adrian and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0909-8836

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

30 Oct 2015 11:29

Last Modified:

25 Jan 2017 12:15

Publisher DOI:

10.1111/eos.12177

PubMed ID:

25782087

Uncontrolled Keywords:

cocamidopropyl betaine, cytotoxicity, dentifrices, sodium lauryl sulfate, toothpaste

BORIS DOI:

10.7892/boris.72328

URI:

https://boris.unibe.ch/id/eprint/72328

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