Efficiency of In Vitro Regeneration is Dependent on the Genotype and Size of Explant in Tef [Eragrostis tef (Zucc.) Trotter]

Plaza-Wüthrich, Sonia; Blösch, Regula; Tadele, Zerihun (2015). Efficiency of In Vitro Regeneration is Dependent on the Genotype and Size of Explant in Tef [Eragrostis tef (Zucc.) Trotter]. Advances in Crop Science and Technology, 3(3), p. 1000179. OMICS International 10.4172/2329-8863.1000179

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Tef [Eragrostis tef (Zucc.) Trotter] is the major cereal crop in the Horn of Africa particularly in Ethiopia where it is staple food for about 50 million people. Its resilience to extreme environmental conditions and high in nutrition makes tef the preferred crop among both farmers and consumers. The efficiency of in vitro regeneration plays significant role in the improvement of crops. We investigated the efficiency of regeneration in 18 tef genotypes (15 landraces and three improved varieties) using three sizes of immature embryos (small, intermediate and large) as an explant. In vitro regeneration was significantly affected by the genotype and the size of the immature embryo used as a donor. Intermediate-size immature embryos which were 101-350 µm long led to the highest percentage of regeneration. Interestingly, the three improved varieties presented very low regeneration efficiencies whereas the landrace Manyi resulted in consistently superior percentage of in vitro regeneration from all three sizes of explants. The findings of this work provide useful insight into the tef germplasm amenable for the regeneration technique which has direct application in techniques such as transformation. It also signifies the importance of using tef landraces instead of improved varieties for in vitro regeneration.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Plaza, Sonia; Blösch, Regula and Tadele, Zerihun

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

2329-8863

Publisher:

OMICS International

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

04 Nov 2015 10:46

Last Modified:

03 May 2016 07:23

Publisher DOI:

10.4172/2329-8863.1000179

Uncontrolled Keywords:

Eragrostis tef, Immature embryo, Plantlet, Regeneration, Embryogenesis, tef

BORIS DOI:

10.7892/boris.72468

URI:

https://boris.unibe.ch/id/eprint/72468

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