The transporter GAT1 plays an important role in GABA-mediated carbon-nitrogen interactions in Arabidopsis

Batushansky, Albert; Kirma, Menny; Grillich, Nicole; Pham, Phuong A.; Rentsch, Doris; Galili, Gad; Fernie, Alisdair R.; Fait, Aaron (2015). The transporter GAT1 plays an important role in GABA-mediated carbon-nitrogen interactions in Arabidopsis. Frontiers in Plant Science, 6(785) Frontiers 10.3389/fpls.2015.00785

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Glutamate derived γ-aminobutyric acid (GABA) is synthetized in the cytosol prior to delivery to the mitochondria where it is catabolized via the TCA cycle. GABA accumulates under various environmental conditions, but an increasing number of studies show its involvement at the crossroad between C and N metabolism. To assess the role of GABA in modulating cellular metabolism, we exposed seedlings of A. thaliana GABA transporter gat1 mutant to full nutrition medium and media deficient in C and N combined with feeding of different concentrations (0.5 and 1 mM) of exogenous GABA. GC-MS based metabolite profiling showed an expected effect of medium composition on the seedlings metabolism of mutant and wild type alike. That being said, a significant interaction between GAT1 deficiency and medium composition was determined with respect to magnitude of change in relative amino acid levels. The effect of exogenous GABA treatment on metabolism was contingent on both the medium and the genotype, leading for instance to a drop in asparagine under full nutrition and low C conditions and glucose under all tested media, but not to changes in GABA content. We additionally assessed the effect of GAT1 deficiency on the expression of glutamate metabolism related genes and genes involved in abiotic stress responses. These results suggest a role for GAT1 in GABA-mediated metabolic alterations in the context of the C-N equilibrium of plant cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology

UniBE Contributor:

Rentsch, Doris

Subjects:

500 Science > 580 Plants (Botany)
500 Science > 570 Life sciences; biology

ISSN:

1664-462X

Publisher:

Frontiers

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

04 Nov 2015 15:45

Last Modified:

04 Nov 2015 15:45

Publisher DOI:

10.3389/fpls.2015.00785

Uncontrolled Keywords:

GABA, glutamate, carbon nitrogen metabolism, GAT1, GC-MS profiling

BORIS DOI:

10.7892/boris.72537

URI:

https://boris.unibe.ch/id/eprint/72537

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