Chlorophyll Breakdown in Senescent Arabidopsis Leaves. Characterization of Chlorophyll Catabolites and of Chlorophyll Catabolic Enzymes Involved in the Degreening Reaction

Pruzinska, Adriana; Tanner, Gaby; Aubry, Silvain; Anders, Iwona; Moser, Simone; Müller, Thomas; Ongania, Karl-Hans; Kräutler, Bernhard; Youn, Li-Young; Liljegren, Sarah J.; Hörtensteiner, Stefan (2005). Chlorophyll Breakdown in Senescent Arabidopsis Leaves. Characterization of Chlorophyll Catabolites and of Chlorophyll Catabolic Enzymes Involved in the Degreening Reaction. Plant Physiology, 139(1), pp. 52-63. American Society of Plant Physiologists 10.1104/pp.105.065870

[img] Text
Plant Physiol.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (471kB) | Request a copy

During senescence, chlorophyll (chl) is metabolized to colorless nonfluorescent chl catabolites (NCCs). A central reaction of the breakdown pathway is the ring cleavage of pheophorbide (pheide) a to a primary fluorescent chl catabolite. Two enzymes catalyze this reaction, pheide a oxygenase (PAO) and red chl catabolite reductase. Five NCCs and three fluorescent chl catabolites (FCCs) accumulated during dark-induced chl breakdown in Arabidopsis (Arabidopsis thaliana). Three of these NCCs and one FCC (primary fluorescent chl catabolite-1) were identical to known catabolites from canola (Brassica napus). The presence in Arabidopsis of two modified FCCs supports the hypothesis that modifications, as present in NCCs, occur at the level of FCC. Chl degradation in Arabidopsis correlated with the accumulation of FCCs and NCCs, as well as with an increase in PAO activity. This increase was due to an up-regulation of Pao gene expression. In contrast, red chl catabolite reductase is not regulated during leaf development and senescence. A pao1 knockout mutant was identified and analyzed. The mutant showed an age- and light-dependent cell death phenotype on leaves and in flowers caused by the accumulation of photoreactive pheide a. In the dark, pao1 exhibited a stay-green phenotype. The key role of PAO in chl breakdown is discussed.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Pruzinska, Adriana; Anders, Iwona and Hörtensteiner, Stefan

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0889

Publisher:

American Society of Plant Physiologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

02 Dec 2015 11:21

Last Modified:

02 Dec 2015 11:21

Publisher DOI:

10.1104/pp.105.065870

BORIS DOI:

10.7892/boris.73234

URI:

https://boris.unibe.ch/id/eprint/73234

Actions (login required)

Edit item Edit item
Provide Feedback