Analytical, physiologic, and clinical validation of a radioimmunoassay for measurement of procollagen type III amino terminal propeptide in serum and bronchoalveolar lavage fluid obtained from dogs.

Schuller, Simone; Valentin, Sophie; Remy, Benoit; Jespers, Pascale; Foulon, Suzanne; Van Israël, Nicole; Clercx, Cécile; McEntee, Kathleen (2006). Analytical, physiologic, and clinical validation of a radioimmunoassay for measurement of procollagen type III amino terminal propeptide in serum and bronchoalveolar lavage fluid obtained from dogs. American journal of veterinary research, 67(5), pp. 749-755. American Veterinary Medical Association 10.2460/ajvr.67.5.749

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OBJECTIVE

To validate a radioimmunoassay for measurement of procollagen type III amino terminal propeptide (PIIINP) concentrations in canine serum and bronchoalveolar lavage fluid (BALF) and investigate the effects of physiologic and pathologic conditions on PIIINP concentrations.

SAMPLE POPULATION

Sera from healthy adult (n = 70) and growing dogs (20) and dogs with chronic renal failure (CRF; 10), cardiomyopathy (CMP; 12), or degenerative valve disease (DVD; 26); and sera and BALF from dogs with chronic bronchopneumopathy (CBP; 15) and healthy control dogs (10 growing and 9 adult dogs).

PROCEDURE

A radioimmunoassay was validated, and a reference range for serum PIIINP (S-PIIINP) concentration was established. Effects of growth, age, sex, weight, CRF, and heart failure on S-PIIINP concentration were analyzed. In CBP-affected dogs, S-PIIINP and BALF-PIIINP concentrations were evaluated.

RESULTS

The radioimmunoassay had good sensitivity, linearity, precision, and reproducibility and reasonable accuracy for measurement of S-PIIINP and BALF-PIIINP concentrations. The S-PIIINP concentration reference range in adult dogs was 8.86 to 11.48 mug/L. Serum PIIINP concentration correlated with weight and age. Growing dogs had significantly higher S-PIIINP concentrations than adults, but concentrations in CRF-, CMP-, DVD-, or CBP-affected dogs were not significantly different from control values. Mean BALF-PIIINP concentration was significantly higher in CBP-affected dogs than in healthy adults.

CONCLUSIONS AND CLINICAL RELEVANCE

In dogs, renal or cardiac disease or CBP did not significantly affect S-PIIINP concentration; dogs with CBP had high BALF-PIIINP concentrations. Data suggest that the use of PIIINP as a marker of pathologic fibrosis might be limited in growing dogs.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Internal Medicine 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
UniBE Contributor:	Schuller, Simone
Subjects:	600 Technology > 610 Medicine & health
ISSN:	0002-9645
Publisher:	American Veterinary Medical Association
Language:	English
Submitter:	Simone Schuller
Date Deposited:	27 Nov 2015 15:27
Last Modified:	05 Dec 2022 14:50
Publisher DOI:	10.2460/ajvr.67.5.749
PubMed ID:	16649905
URI:	https://boris.unibe.ch/id/eprint/73245