Arabidopsis AXR6 encodes CUL1 implicating SCF E3 ligases in auxin regulation of embryogenesis

Hellmann, H.; Hobbie, Lawrence; Chapman, Anngela; Dharmasiri, Sunethra; Dharmasiri, Nihal; del Pozo, Carlos; Reinhardt, Didier; Estelle, Mark (2003). Arabidopsis AXR6 encodes CUL1 implicating SCF E3 ligases in auxin regulation of embryogenesis. The EMBO Journal, 22(13), pp. 3314-3325. EMBO Press 10.1093/emboj/cdg335

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The AXR6 gene is required for auxin signaling in the Arabidopsis embryo and during postembryonic development. One of the effects of auxin is to stimulate degradation of the Aux/IAA auxin response proteins through the action of the ubiquitin protein ligase SCFTIR1. Here we show that AXR6 encodes the SCF subunit CUL1. The axr6 mutations affect the ability of mutant CUL1 to assemble into stable SCF complexes resulting in reduced degradation of the SCFTIR1 substrate AXR2/IAA7. In addition, we show that CUL1 is required for lateral organ initiation in the shoot apical meristem and the inflorescence meristem. These results indicate that the embryonic axr6 phenotype is related to a defect in SCF function and accumulation of Aux/IAA proteins such as BDL/IAA12. In addition, we show that CUL1 has a role in auxin response throughout the life cycle of the plant.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Reinhardt, Didier

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1460-2075

Publisher:

EMBO Press

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

07 Dec 2015 10:53

Last Modified:

07 Dec 2015 10:53

Publisher DOI:

10.1093/emboj/cdg335

Uncontrolled Keywords:

Arabidopsis, Auxin, Cullin, IAASCF

BORIS DOI:

10.7892/boris.73359

URI:

https://boris.unibe.ch/id/eprint/73359

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