Search for Low-Molecular-Weight Biomarkers in Plant Tissues and Seeds Using Metabolomics: Tools, Strategies, and Applications

Marti, Guillaume; Erb, Matthias; Rudaz, Serge; Turlings, Ted; Wolfender, Jean-Luc (2012). Search for Low-Molecular-Weight Biomarkers in Plant Tissues and Seeds Using Metabolomics: Tools, Strategies, and Applications. In: Agrawal, Ganehs K.; Rakwal, Randeep (eds.) Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield (pp. 305-341). Springer Netherlands 10.1007/978-94-007-4749-4_16

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This chapter summarises the metabolomic strategies currently in force used in plant science and describes the methods used. The metabolite profiling and fingerprinting of plant tissues through MS- and/or NMR-based approaches and the subsequent identification of biomarkers is detailed. Strategies for the microisolation and de novo identification of unknown biomarkers are also discussed. The various approaches are illustrated by a metabolomic study of the maize response to herbivory. A review of recent metabolomic studies performed on seed and crop plant tissues involving various analytical strategies is provided.

Item Type:

Book Section (Book Chapter)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Erb, Matthias

Subjects:

500 Science > 580 Plants (Botany)

ISBN:

978-94-007-4748-7

Publisher:

Springer Netherlands

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

02 Dec 2015 11:48

Last Modified:

02 Dec 2015 11:51

Publisher DOI:

10.1007/978-94-007-4749-4_16

Uncontrolled Keywords:

Metabolomics; Biomarker identification; Liquid chromatography; Mass spectrometry; Nuclear magnetic resonance; Crop plants

BORIS DOI:

10.7892/boris.73376

URI:

https://boris.unibe.ch/id/eprint/73376

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