DNA Catalysis: The Chemical Repertoire of DNAzymes

Hollenstein, Marcel (2015). DNA Catalysis: The Chemical Repertoire of DNAzymes. Molecules, 20(11), pp. 20777-20804. Molecular Diversity Preservation International MDPI 10.3390/molecules201119730

[img]
Preview
Text
Molecules2015(DNAzymes).pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (5MB) | Preview

Deoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are obtained by combinatorial in vitro selection methods. Initially conceived to function as gene silencing agents, the scope of DNAzymes has rapidly expanded into diverse fields, including biosensing, diagnostics, logic gate operations, and the development of novel synthetic and biological tools. In this review, an overview of all the different chemical reactions catalyzed by DNAzymes is given with an emphasis on RNA cleavage and the use of non-nucleosidic substrates. The use of modified nucleoside triphosphates (dN*TPs) to expand the chemical space to be explored in selection experiments and ultimately to generate DNAzymes with an expanded chemical repertoire is also highlighted.

Item Type:

Journal Article (Review Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Hollenstein, Marcel

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1420-3049

Publisher:

Molecular Diversity Preservation International MDPI

Funders:

[4] Swiss National Science Foundation

Language:

English

Submitter:

Marcel Hollenstein

Date Deposited:

09 Dec 2015 08:43

Last Modified:

11 Sep 2017 08:34

Publisher DOI:

10.3390/molecules201119730

BORIS DOI:

10.7892/boris.73559

URI:

https://boris.unibe.ch/id/eprint/73559

Actions (login required)

Edit item Edit item
Provide Feedback