Qualitative Distinction of Autotrophic and Heterotrophic Processes at the Leaf Level by Means of Triple Stable Isotope (C–O–H) Patterns

Kimak, Adam; Kern, Zoltan; Leuenberger, Markus (2015). Qualitative Distinction of Autotrophic and Heterotrophic Processes at the Leaf Level by Means of Triple Stable Isotope (C–O–H) Patterns. Frontiers in Plant Science, 6 Frontiers 10.3389/fpls.2015.01008

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Foliar samples were harvested from two oaks, a beech, and a yew at the same site in order to trace the development of the leaves over an entire vegetation season. Cellulose yield and stable isotopic compositions (δ13C, δ18O, and δD) were analyzed on leaf cellulose. All parameters unequivocally define a juvenile and a mature period in the foliar expansion of each species. The accompanying shifts of the δ13C-values are in agreement with the transition from remobilized carbohydrates (juvenile period), to current photosynthates (mature phase). While the opponent seasonal trends of δ18O of blade and vein cellulose are in perfect agreement with the state-of-art mechanistic understanding, the lack of this discrepancy for δD, documented for the first time, is unexpected. For example, the offset range of 18 permil (oak veins) to 57 permil (oak blades) in δD may represent a process driven shift from autotrophic to heterotrophic processes. The shared pattern between blade and vein found for both oak and beech suggests an overwhelming metabolic isotope effect on δD that might be accompanied by proton transfer linked to the Calvin-cycle. These results provide strong evidence that hydrogen and oxygen are under different biochemical controls even at the leaf level.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > Oeschger Centre for Climate Change Research (OCCR)
08 Faculty of Science > Physics Institute > Climate and Environmental Physics

UniBE Contributor:

Kimak, Adam and Leuenberger, Markus

Subjects:

500 Science > 530 Physics
500 Science > 550 Earth sciences & geology

ISSN:

1664-462X

Publisher:

Frontiers

Language:

English

Submitter:

Doris Rätz

Date Deposited:

08 Dec 2015 10:25

Last Modified:

08 Dec 2015 10:25

Publisher DOI:

10.3389/fpls.2015.01008

BORIS DOI:

10.7892/boris.73810

URI:

https://boris.unibe.ch/id/eprint/73810

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