Analytic validation of a gas chromatography-mass spectrometry method for quantification of six amino acids in canine serum samples.

Lopes, Rosana; Grützner, Niels; Berghoff, Nora; Lidbury, Jonathan A; Suchodolski, Jan S; Steiner, Jörg M (2015). Analytic validation of a gas chromatography-mass spectrometry method for quantification of six amino acids in canine serum samples. American journal of veterinary research, 76(12), pp. 1014-1021. American Veterinary Medical Association 10.2460/ajvr.76.12.1014

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OBJECTIVE To analytically validate a gas concentration of chromatography-mass spectrometry (GC-MS) method for measurement of 6 amino acids in canine serum samples and to assess the stability of each amino acid after sample storage. SAMPLES Surplus serum from 80 canine samples submitted to the Gastrointestinal Laboratory at Texas A&M University and serum samples from 12 healthy dogs. PROCEDURES GC-MS was validated to determine precision, reproducibility, limit of detection, and percentage recovery of known added concentrations of 6 amino acids in surplus serum samples. Amino acid concentrations in serum samples from healthy dogs were measured before (baseline) and after storage in various conditions. RESULTS Intra- and interassay coefficients of variation (10 replicates involving 12 pooled serum samples) were 13.4% and 16.6% for glycine, 9.3% and 12.4% for glutamic acid, 5.1% and 6.3% for methionine, 14.0% and 15.1% for tryptophan, 6.2% and 11.0% for tyrosine, and 7.4% and 12.4% for lysine, respectively. Observed-to-expected concentration ratios in dilutional parallelism tests (6 replicates involving 6 pooled serum samples) were 79.5% to 111.5% for glycine, 80.9% to 123.0% for glutamic acid, 77.8% to 111.0% for methionine, 85.2% to 98.0% for tryptophan, 79.4% to 115.0% for tyrosine, and 79.4% to 110.0% for lysine. No amino acid concentration changed significantly from baseline after serum sample storage at -80°C for ≤ 7 days. CONCLUSIONS AND CLINICAL RELEVANCE GC-MS measurement of concentration of 6 amino acids in canine serum samples yielded precise, accurate, and reproducible results. Sample storage at -80°C for 1 week had no effect on GC-MS results.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Swine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management

UniBE Contributor:

Grützner, Niels

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
600 Technology > 610 Medicine & health

ISSN:

0002-9645

Publisher:

American Veterinary Medical Association

Language:

English

Submitter:

Daniela Fasel

Date Deposited:

10 Dec 2015 15:18

Last Modified:

10 Sep 2017 18:37

Publisher DOI:

10.2460/ajvr.76.12.1014

PubMed ID:

26618725

BORIS DOI:

10.7892/boris.74081

URI:

https://boris.unibe.ch/id/eprint/74081

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