Osseointegration of biochemically modified implants in an osteoporosis rodent model

Stadlinger, B.; Korn, P.; Tödtmann, N.; Eckelt, U.; Range, U.; Bürki, A.; Ferguson, S. J.; Kramer, I.; Kautz, A.; Schnabelrauch, M.; Kneissel, M.; Schlottig, F. (2013). Osseointegration of biochemically modified implants in an osteoporosis rodent model. European cells & materials eCM, 25, pp. 326-340. University of Wales

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The present study examined the impact of implant surface modifications on osseointegration in an osteoporotic rodent model. Sandblasted, acid-etched titanium implants were either used directly (control) or were further modified by surface conditioning with NaOH or by coating with one of the following active agents: collagen/chondroitin sulphate, simvastatin, or zoledronic acid. Control and modified implants were inserted into the proximal tibia of aged ovariectomised (OVX) osteoporotic rats (n = 32/group). In addition, aged oestrogen competent animals received either control or NaOH conditioned implants. Animals were sacrificed 2 and 4 weeks post-implantation. The excised tibiae were utilised for biomechanical and morphometric readouts (n = 8/group/readout). Biomechanical testing revealed at both time points dramatically reduced osseointegration in the tibia of oestrogen deprived osteoporotic animals compared to intact controls irrespective of NaOH exposure. Consistently, histomorphometric and microCT analyses demonstrated diminished bone-implant contact (BIC), peri-implant bone area (BA), bone volume/tissue volume (BV/TV) and bone-mineral density (BMD) in OVX animals. Surface coating with collagen/chondroitin sulphate had no detectable impact on osseointegration. Interestingly, statin coating resulted in a transient increase in BIC 2 weeks post-implantation; which, however, did not correspond to improvement of biomechanical readouts. Local exposure to zoledronic acid increased BIC, BA, BV/TV and BMD at 4 weeks. Yet this translated only into a non-significant improvement of biomechanical properties. In conclusion, this study presents a rodent model mimicking severely osteoporotic bone. Contrary to the other bioactive agents, locally released zoledronic acid had a positive impact on osseointegration albeit to a lesser extent than reported in less challenging models.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB

UniBE Contributor:

Bürki, Alexander

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1473-2262

Publisher:

University of Wales

Language:

English

Submitter:

Alexander Bürki

Date Deposited:

15 Dec 2015 09:55

Last Modified:

16 Dec 2015 15:39

PubMed ID:

23832686

BORIS DOI:

10.7892/boris.74291

URI:

https://boris.unibe.ch/id/eprint/74291

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