Molecular profiling of lung adenosquamous carcinoma: hybrid or genuine type?

Vassella, Erik; Langsch, Stephanie; Dettmer, Matthias; Schlup, Cornelia; Neuenschwander, Maja; Frattini, Milo; Gugger, Mathias; Schäfer, Stephan C. (2015). Molecular profiling of lung adenosquamous carcinoma: hybrid or genuine type? OncoTarget, 6(27), pp. 23905-23916. Impact Journals LLC 10.18632/oncotarget.4163

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Lung adenosquamous carcinoma is a particular subtype of non-small cell lung carcinoma that is defined by the coexistence of adenocarcinoma and squamous cell carcinoma components. The aim of this study was to assess the mutational profile in each component of 16 adenosquamous carcinoma samples from a Caucasian population by a combination of next generation sequencing using the cancer hotspot panel as well as the colon and lung cancer panel and FISH. Identified mutations were confirmed by Sanger sequencing of DNA from cancer cells of each component collected by Laser Capture microdissection. Mutations typical for adenocarcinoma as well as squamous cell carcinoma were identified. Driver mutations were predominantly in the trunk suggesting a monoclonal origin of adenosquamous carcinoma. Most remarkably, EGFR mutations and mutations in the PI3K signaling pathway, which accounted for 30% and 25% of tumors respectively, were more prevalent while KRAS mutations were less prevalent than expected for a Caucasian population. Surprisingly, expression of classifier miR-205 was intermediate between that of classical adenocarcinoma and squamous cell carcinoma suggesting that adenosquamous carcinoma is a transitional stage between these tumor types. The high prevalence of therapy-relevant targets opens new options of therapeutic intervention for adenosquamous carcinoma patients.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Pathology > Clinical Pathology
04 Faculty of Medicine > Service Sector > Institute of Pathology

UniBE Contributor:

Vassella, Erik; Langsch, Stephanie; Dettmer, Matthias; Schlup, Cornelia; Neuenschwander, Maja; Frattini, Milo; Gugger, Mathias and Schäfer, Stephan

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1949-2553

Publisher:

Impact Journals LLC

Language:

English

Submitter:

Doris Haefelin

Date Deposited:

17 Dec 2015 10:49

Last Modified:

15 Nov 2017 07:47

Publisher DOI:

10.18632/oncotarget.4163

PubMed ID:

26068980

Uncontrolled Keywords:

adenosquamous carcinoma lung microRNA next-generation sequencing therapy-relevant mutation

BORIS DOI:

10.7892/boris.74341

URI:

https://boris.unibe.ch/id/eprint/74341

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