König, Stefan; Aebi, Beat; Lanz, Stephan; Gasser, Martina; Weinmann, Wolfgang (2011). On-line SPE LC-MS/MS for the quantification of Δ9-tetrahydrocannabinol (THC) and its two major metabolites in human peripheral blood by liquid chromatography tandem mass spectrometry. Analytical and bioanalytical chemistry, 400(1), pp. 9-16. Heidelberg: Springer-Verlag 10.1007/s00216-011-4708-x
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A universal and robust analytical method for the determination of Δ9-tetrahydrocannabinol (THC) and two of its metabolites Δ9-(11-OH)-tetrahydrocannabinol (11-OH-THC) and 11-nor-Δ9-carboxy-tetrahydrocannabinol (THC-COOH) in human whole blood was developed and validated for use in forensic toxicology. Protein precipitation, integrated solid phase extraction and on-line enrichment followed by high-performance liquid chromatography separation and detection with a triple quadrupole mass spectrometer were combined. The linear ranges used for the three cannabinoids were from 0.5 to 20 ng/mL for THC and 11-OH-THC and from 2.5 to 100 ng/mL for THC-COOH, therefore covering the requirements for forensic use. Correlation coefficients of 0.9980 or better were achieved for all three analytes. No relevant hydrolysis was observed for THC-COOH glucuronide with this procedure--in contrast to our previous GC-MS procedure, which obviously lead to an artificial increase of the THC-COOH concentration due to the hydrolysis of the glucuronide-conjugate occurring at high pH during the phase-transfer catalyzed methylation step.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Chemistry and Toxicology 04 Faculty of Medicine > Service Sector > Institute of Legal Medicine |
UniBE Contributor: |
König, Stefan (B), Lanz, Stephan, Gasser, Martina Sandra, Weinmann, Wolfgang |
ISSN: |
1618-2642 |
Publisher: |
Springer-Verlag |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:22 |
Last Modified: |
29 Mar 2023 23:32 |
Publisher DOI: |
10.1007/s00216-011-4708-x |
PubMed ID: |
21311875 |
Web of Science ID: |
000288658800002 |
BORIS DOI: |
10.7892/boris.7447 |
URI: |
https://boris.unibe.ch/id/eprint/7447 (FactScience: 212709) |
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