Staphylococcus aureus genotype B and other genotypes isolated from cow milk in European countries

Cosandey, Aurelie; Boss, R; Luini, M; Artursson, K; Bardiau, M; Breitenwieser, F; Hehenberger, Elisabeth Maria; Lam, Th; Mansfeld, M; Michel, Astrid; Mösslacher, G; Naskova, J; Nelson, S; Podpečan, O; Raemy, Andreas; Ryan, E; Salat, O; Zangerl, P; Steiner, Adrian and Graber, H U (2015). Staphylococcus aureus genotype B and other genotypes isolated from cow milk in European countries. Journal of dairy science, 99(1), pp. 529-540. American Dairy Science Association 10.3168/jds.2015-9587

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Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies, however, have demonstrated in Swiss cows that Staph. aureus isolated from bovine intramammary infection is genetically heterogeneous, with Staph. aureus genotype B (GTB) and GTC being the most prominent genotypes. In addition, Staph. aureus GTB was found to be contagious, whereas Staph. aureus GTC and all the remaining genotypes were involved in individual cow disease. The aim of this study was to subtype strains of Staph. aureus isolated from bovine mastitic milk and bulk tank milk to obtain a unified view of the presence of bovine staphylococcal subtypes in 12 European countries. A total of 456 strains of Staph. aureus were subjected to different typing methods: ribosomal spacer PCR, detection of enterotoxin genes, and detection of gene polymorphisms (lukE, coa). Major genotypes with their variants were combined into genotypic clusters (CL). This study revealed 5 major CL representing 76% of all strains and comprised CLB, CLC, CLF, CLI, and CLR. The clusters were characterized by the same genetic properties as the Swiss isolates, demonstrating high clonality of bovine Staph. aureus. Interestingly, CLB was situated in central Europe whereas the other CL were widely disseminated. The remaining 24% of the strains comprised 41 genotypes and variants, some of which (GTAM, GTBG) were restricted to certain countries; many others, however, were observed only once.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management

UniBE Contributor:

Cosandey, Aurelie; Hehenberger, Elisabeth Maria; Michel, Astrid; Raemy, Andreas and Steiner, Adrian

Subjects:

600 Technology > 630 Agriculture
500 Science > 590 Animals (Zoology)
600 Technology > 610 Medicine & health

ISSN:

0022-0302

Publisher:

American Dairy Science Association

Language:

English

Submitter:

Patrik Zanolari

Date Deposited:

11 Jan 2016 11:44

Last Modified:

18 Jan 2016 11:25

Publisher DOI:

10.3168/jds.2015-9587

PubMed ID:

26585469

Uncontrolled Keywords:

Staphylococcus aureus; bovine intramammary infection; genotyping; virulence gene; Europe

BORIS DOI:

10.7892/boris.74486

URI:

https://boris.unibe.ch/id/eprint/74486

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