Jousset, Florian; Rohr, Stephan (2015). Optical recording of calcium currents during impulse conduction in cardiac tissue. Neurophotonics, 2(2), 021011. SPIE 10.1117/1.NPh.2.2.021011
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We explore the feasibility of obtaining a spatially resolved picture of Ca2+Ca2+ inward currents (ICaICa) in multicellular cardiac tissue by differentiating optically recorded Ca2+Ca2+ transients that accompany propagating action potentials. Patterned growth strands of neonatal rat ventricular cardiomyocytes were stained with the Ca2+Ca2+ indicators Fluo-4 or Fluo-4FF. Preparations were stimulated at 1 Hz, and Ca2+Ca2+ transients were recorded with high spatiotemporal resolution (50 μm50 μm, 2 kHz analog bandwidth) with a photodiode array. Signals were differentiated after appropriate digital filtering. Differentiation of Ca2+Ca2+ transients resulted in optically recorded calcium currents (ORCCs) that carried the temporal and pharmacological signatures of L-type Ca2+Ca2+ inward currents: the time to peak amounted to ∼2.1 ms∼2.1 ms (Fluo-4FF) and ∼2.4 ms∼2.4 ms (Fluo-4), full-width at half-maximum was ∼8 ms∼8 ms, and ORCCs were completely suppressed by 50 μmol/L50 μmol/LCdCl2CdCl2. Also, and as reported before from patch-clamp studies, caffeine reversibly depressed the amplitude of ORCCs. The results demonstrate that the differentiation of Ca2+Ca2+ transients can be used to obtain a spatially resolved picture of the initial phase of ICaICa in cardiac tissue and to assess relative changes of activation/fast inactivation of ICaICa following pharmacological interventions.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Physiology |
UniBE Contributor: |
Jousset, Florian, Rohr, Stephan |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2329-423X |
Publisher: |
SPIE |
Language: |
English |
Submitter: |
Stefan von Känel-Zimmermann |
Date Deposited: |
13 Jan 2016 13:55 |
Last Modified: |
05 Dec 2022 14:50 |
Publisher DOI: |
10.1117/1.NPh.2.2.021011 |
BORIS DOI: |
10.7892/boris.74553 |
URI: |
https://boris.unibe.ch/id/eprint/74553 |
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