Luminal particles within cellular microtubules.

Garvalov, Boyan K; Zuber, Benoît; Bouchet-Marquis, Cédric; Kudryashev, Mikhail; Gruska, Manuela; Beck, Martin; Leis, Andrew; Frischknecht, Friedrich; Bradke, Frank; Baumeister, Wolfgang; Dubochet, Jacques; Cyrklaff, Marek (2006). Luminal particles within cellular microtubules. Journal of cell biology, 174(6), pp. 759-765. Rockefeller Institute Press 10.1083/jcb.200606074

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The regulation of microtubule dynamics is attributed to microtubule-associated proteins that bind to the microtubule outer surface, but little is known about cellular components that may associate with the internal side of microtubules. We used cryoelectron tomography to investigate in a quantitative manner the three dimensional structure of microtubules in intact mammalian cells. We show that the lumen of microtubules in this native state is filled with discrete, globular particles with a diameter of 7 nm and spacings between 8 and 20 nm in neuronal cells. Cross-sectional views of microtubules confirm the presence of luminal material in vitreous sections of brain tissue. Most of the luminal particles had connections to the microtubule wall, as revealed in tomograms. A higher accumulation of particles was seen near the retracting plus ends of microtubules. The luminal particles were abundant in neurons, but were also observed in other cells, such as astrocytes and stem cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Zuber, Benoît

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0021-9525

Publisher:

Rockefeller Institute Press

Language:

English

Submitter:

Benoît Zuber

Date Deposited:

28 Jan 2016 15:19

Last Modified:

28 Jan 2016 15:19

Publisher DOI:

10.1083/jcb.200606074

PubMed ID:

16954350

BORIS DOI:

10.7892/boris.74797

URI:

https://boris.unibe.ch/id/eprint/74797

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