Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation.

Salje, Jeanne; Zuber, Benoît; Löwe, Jan (2009). Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation. Science, 323(5913), pp. 509-512. American Association for the Advancement of Science 10.1126/science.1164346

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Bipolar elongation of filaments of the bacterial actin homolog ParM drives movement of newly replicated plasmid DNA to opposite poles of a bacterial cell. We used a combination of vitreous sectioning and electron cryotomography to study this DNA partitioning system directly in native, frozen cells. The diffraction patterns from overexpressed ParM bundles in electron cryotomographic reconstructions were used to unambiguously identify ParM filaments in Escherichia coli cells. Using a low-copy number plasmid encoding components required for partitioning, we observed small bundles of three to five intracellular ParM filaments that were situated close to the edge of the nucleoid. We propose that this may indicate the capture of plasmid DNA within the periphery of this loosely defined, chromosome-containing region.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Zuber, Benoît

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0036-8075

Publisher:

American Association for the Advancement of Science

Language:

English

Submitter:

Benoît Zuber

Date Deposited:

28 Jan 2016 15:07

Last Modified:

31 Jan 2016 16:25

Publisher DOI:

10.1126/science.1164346

PubMed ID:

19095899

BORIS DOI:

10.7892/boris.74803

URI:

https://boris.unibe.ch/id/eprint/74803

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