Direct observation of molecular arrays in the organized smooth endoplasmic reticulum.

Korkhov, Vladimir M; Zuber, Benoît (2009). Direct observation of molecular arrays in the organized smooth endoplasmic reticulum. BMC cell biology, 10(59), p. 59. BioMed Central 10.1186/1471-2121-10-59

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BACKGROUND

Tubules and sheets of endoplasmic reticulum perform different functions and undergo inter-conversion during different stages of the cell cycle. Tubules are stabilized by curvature inducing resident proteins, but little is known about the mechanisms of endoplasmic reticulum sheet stabilization. Tethering of endoplasmic reticulum membranes to the cytoskeleton or to each other has been proposed as a plausible way of sheet stabilization.

RESULTS

Here, using fluorescence microscopy we show that the previously proposed mechanisms, such as membrane tethering via GFP-dimerization or coiled coil protein aggregation do not explain the formation of the calnexin-induced organized smooth endoplasmic reticulum membrane stacks. We also show that the LINC complex proteins known to serve a tethering function in the nuclear envelope are excluded from endoplasmic reticulum stacks. Finally, using cryo-electron microscopy of vitreous sections methodology that preserves cellular architecture in a hydrated, native-like state, we show that the sheet stacks are highly regular and may contain ordered arrays of macromolecular complexes. Some of these complexes decorate the cytosolic surface of the membranes, whereas others appear to span the width of the cytosolic or luminal space between the stacked sheets.

CONCLUSION

Our results provide evidence in favour of the hypothesis of endoplasmic reticulum sheet stabilization by intermembrane tethering.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Zuber, Benoît

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1471-2121

Publisher:

BioMed Central

Language:

English

Submitter:

Benoît Zuber

Date Deposited:

22 Jan 2016 15:24

Last Modified:

28 Nov 2020 02:26

Publisher DOI:

10.1186/1471-2121-10-59

PubMed ID:

19703297

BORIS DOI:

10.7892/boris.74804

URI:

https://boris.unibe.ch/id/eprint/74804

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