Polydnavirus DNA of the braconid wasp Chelonus inanitus is integrated in the wasp's genome and excised only in later pupal and adult stages of the female

Gruber, Andreas; Stettler, P; Heiniger, P; Schümperli, Daniel; Lanzrein, B (1996). Polydnavirus DNA of the braconid wasp Chelonus inanitus is integrated in the wasp's genome and excised only in later pupal and adult stages of the female. Journal of general virology, 77(11), pp. 2873-2879. Society for General Microbiology 10.1099/0022-1317-77-11-2873

[img] Text
gruber 1996.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (2MB) | Request a copy

Many endoparasitic wasps inject, along with the egg, polydnavirus into their insect hosts, the virus being a prerequisite for successful parasitoid development. The genome of polydnaviruses consists of multiple circular dsDNA molecules of variable size. We show for a 12 kbp segment of the braconid Chelonus inanitus (CiV12) that it is integrated into the wasp genome. This is the first direct demonstration of integration for a bracovirus. PCR data indicated that the integrated form of CiV12 was present in all male and female stages investigated while the excised circular virus DNA only appeared in females after a specific stage in pupal-adult development. The data also indicated that after excision of virus DNA the genomic DNA was rejoined. This has not yet been reported for any polydnavirus. Sequence analyses in the junction regions revealed the presence of an imperfect consensus sequence of 15 nucleotides in CiV12, in each terminus of the integrated virus DNA and in the rejoined genomic DNA. Within these repeats two sequence types (ATA, TAC) were observed in the various virus clones and in the clones encompassing the rejoined genomic DNA; they corresponded to the sequence type in the right and left junction, respectively. To explain this, we propose a model of virus DNA replication in which the genomic DNA is folded to juxtapose the direct repeat of the left with that of the right junction; recombination at specific sites would then yield the two types of virus and rejoined genomic DNA.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Gruber, Andreas and Schümperli, Daniel

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0022-1317

Publisher:

Society for General Microbiology

Language:

English

Submitter:

Andreas Gruber

Date Deposited:

28 Jan 2016 15:29

Last Modified:

11 Sep 2017 19:04

Publisher DOI:

10.1099/0022-1317-77-11-2873

PubMed ID:

8922483

BORIS DOI:

10.7892/boris.74823

URI:

https://boris.unibe.ch/id/eprint/74823

Actions (login required)

Edit item Edit item
Provide Feedback