Low-cost disposable ALT electrochemical microsensors for in-vitro hepatotoxic assessment

la Cour, Jakob B.; Generelli, Silvia; Barbe, Laurent; Guenat, Olivier Thierry (2016). Low-cost disposable ALT electrochemical microsensors for in-vitro hepatotoxic assessment. Sensors and Actuators B: Chemical, 228, pp. 360-365. Elsevier 10.1016/j.snb.2016.01.008

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Liver-on-chip systems are widely seen as having the potential to replace animal testing for long-term liver toxicity assessments. However, such systems necessitate solutions, such as electrochemical microsensors, to provide information about the cells exposed to chemical compounds in a confined space. This study describes the development of microsensors for the detection of alanine-aminotransferase (ALT), an intracellular enzyme found in hepatocytes, for monitoring the viability of in-vitro hepatic cell cultures. The electrochemical sensors were developed by using screen printed electrodes functionalized by drop-casting. These technologies are intended to produce disposable and low-cost sensors that can easily be exchanged once their performance is degraded. The sensors are capable of measuring ALT in a microfluidic environment through the detection of changes in glutamate concentration. The microsensors were found to be stable for more than 60 days and were successfully tested using hepatocellular lysates to assess their capability to quantify ALT activity in a hepatic cell culture. These results open the way to their integration in liver bioreactors to assess hepatocellular toxicity in-vitro.

Item Type:

Journal Article (Original Article)

Division/Institute:

10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Lung Regeneration Technologies
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology

UniBE Contributor:

Guenat, Olivier Thierry

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 620 Engineering

ISSN:

0925-4005

Publisher:

Elsevier

Language:

English

Submitter:

Olivier Thierry Guenat

Date Deposited:

18 Jul 2016 12:23

Last Modified:

21 Jul 2016 20:01

Publisher DOI:

10.1016/j.snb.2016.01.008

BORIS DOI:

10.7892/boris.75108

URI:

https://boris.unibe.ch/id/eprint/75108

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