Quantitative PCR for the diagnosis of cutaneous leishmaniasis from formalin-fixed and paraffin-embedded skin sections.

Müller, Norbert; Hentrich, Brigitte; Frey, Caroline; Welle, Monika Maria (2015). Quantitative PCR for the diagnosis of cutaneous leishmaniasis from formalin-fixed and paraffin-embedded skin sections. Molecular and cellular probes, 29(6), pp. 507-510. Elsevier 10.1016/j.mcp.2015.09.008

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The present report describes a real-time PCR-based procedure to reliably determine the quantity of Leishmania amastigotes in relation to the amount of host tissue in histological skin sections from canine and equine cases of cutaneous leishmaniasis. The novel diagnostic Leishmania-PCR has a detection limit of <0.02 amastigotes per μg tissue, which corresponds well to the detection limit of immunohistochemistry and is far beyond that of conventional histology. Our results emphasise the importance of PCR to complement routine histology of cutaneous leishmaniasis cases, particularly in laboratories in which no immunohistochemical assay is available.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Müller, Norbert; Hentrich, Brigitte; Frey, Caroline and Welle, Monika Maria

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0890-8508

Publisher:

Elsevier

Language:

English

Submitter:

Caroline Franziska Frey Marreros Canales

Date Deposited:

02 Feb 2016 08:43

Last Modified:

01 Oct 2016 02:30

Publisher DOI:

10.1016/j.mcp.2015.09.008

PubMed ID:

26427730

Uncontrolled Keywords:

Cutaneous leishmaniasis; Diagnosis; Histology; Leishmania spp.; Quantitative real-time PCR

BORIS DOI:

10.7892/boris.75146

URI:

https://boris.unibe.ch/id/eprint/75146

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