Classical swine fever virus replicon particles lacking the Erns gene: a potential marker vaccine for intradermal application.

Frey, Caroline; Bauhofer, Oliver; Ruggli, Nicolas; Summerfield, Artur; Hofmann, Martin A; Tratschin, Jon-Duri (2006). Classical swine fever virus replicon particles lacking the Erns gene: a potential marker vaccine for intradermal application. Veterinary research, 37(5), pp. 655-670. Editions scientifiques Elsevier 10.1051/vetres:2006028

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Classical swine fever virus replicon particles (CSF-VRP) deficient for E(rns) were evaluated as a non-transmissible marker vaccine. A cDNA clone of CSFV strain Alfort/187 was used to obtain a replication-competent mutant genome (replicon) lacking the sequence encoding the 227 amino acids of the glycoprotein E(rns) (A187delE(rns)). For packaging of A187delE(rns) into virus particles, porcine kidney cell lines constitutively expressing E(rns) of CSFV were established. The rescued VRP were infectious in cell culture but did not yield infectious progeny virus. Single intradermal vaccination of two pigs with 10(7) TCID(50) of VRP A187delE(rns) elicited neutralizing antibodies, anti-E2 antibodies, and cellular immune responses determined by an increase of IFN-gamma producing cells. No anti-E(rns) antibodies were detected in the vaccinees confirming that this vaccine represents a negative marker vaccine allowing differentiation between infected and vaccinated animals. The two pigs were protected against lethal challenge with the highly virulent CSFV strain Eystrup. In contrast, oral immunization resulted in only partial protection, and neither CSFV-specific antibodies nor stimulated T-cells were found before challenge. These data represent a good basis for more extended vaccination/challenge trials including larger numbers of animals as well as more thorough analysis of virus shedding using sentinel animals to monitor horizontal spread of the challenge virus.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Frey, Caroline; Ruggli, Nicolas and Summerfield, Artur

Subjects:

600 Technology > 630 Agriculture

ISSN:

0928-4249

Publisher:

Editions scientifiques Elsevier

Language:

English

Submitter:

Caroline Franziska Frey Marreros Canales

Date Deposited:

01 Feb 2016 16:52

Last Modified:

01 Feb 2016 16:52

Publisher DOI:

10.1051/vetres:2006028

PubMed ID:

16777037

BORIS DOI:

10.7892/boris.75150

URI:

https://boris.unibe.ch/id/eprint/75150

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