Development of a rapid column-switching LC-MS/MS method for the quantification of THCCOOH and THCCOOH-glucuronide in whole blood for assessing cannabis consumption frequency

Hädener, Marianne; Weinmann, Wolfgang; Schürch, Stefan; König, Stefan (2016). Development of a rapid column-switching LC-MS/MS method for the quantification of THCCOOH and THCCOOH-glucuronide in whole blood for assessing cannabis consumption frequency. Analytical and bioanalytical chemistry, 408(7), pp. 1953-1962. Springer 10.1007/s00216-016-9311-8

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The concentration of 11-nor-9-carboxy-Δ(9)-tetrahydrocannabinol (THCCOOH) in whole blood is used as a parameter for assessing the consumption behavior of cannabis consumers. The blood level of THCCOOH-glucuronide might provide additional information about the frequency of cannabis use. To verify this assumption, a column-switching liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the rapid and direct quantification of free and glucuronidated THCCOOH in human whole blood was newly developed. The method comprised protein precipitation, followed by injection of the processed sample onto a trapping column and subsequent gradient elution to an analytical column for separation and detection. The total LC run time was 4.5 min. Detection of the analytes was accomplished by electrospray ionization in positive ion mode and selected reaction monitoring using a triple-stage quadrupole mass spectrometer. The method was fully validated by evaluating the following parameters: linearity, lower limit of quantification, accuracy and imprecision, selectivity, extraction efficiency, matrix effect, carry-over, dilution integrity, analyte stability, and re-injection reproducibility. All acceptance criteria were analyzed and the predefined criteria met. Linearity ranged from 5.0 to 500 μg/L for both analytes. The method was successfully applied to whole blood samples from a large collective of cannabis consumers, demonstrating its applicability in the forensic field.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Chemistry and Toxicology
08 Faculty of Science > Departement of Chemistry and Biochemistry
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine

UniBE Contributor:

Hädener, Marianne; Weinmann, Wolfgang; Schürch, Stefan and König, Stefan

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
600 Technology > 610 Medicine & health

ISSN:

1618-2642

Publisher:

Springer

Language:

English

Submitter:

Antoinette Angehrn

Date Deposited:

04 Feb 2016 08:10

Last Modified:

13 Oct 2017 12:48

Publisher DOI:

10.1007/s00216-016-9311-8

PubMed ID:

26781107

Uncontrolled Keywords:

Column-switching chromatography; DUI; LC-MS/MS; THCCOOH; THCCOOH-glucuronide; Whole blood

BORIS DOI:

10.7892/boris.75433

URI:

https://boris.unibe.ch/id/eprint/75433

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