Conditioned medium from fresh and demineralized bone enhances osteoclastogenesis in murine bone marrow cultures

Caballé Serrano, Jordi; Schuldt Filho, Guenther; Bosshardt, Dieter; Gargallo-Albiol, Jordi; Buser, Daniel; Gruber, Reinhard (2016). Conditioned medium from fresh and demineralized bone enhances osteoclastogenesis in murine bone marrow cultures. Clinical oral implants research, 27(2), pp. 226-232. Wiley-Blackwell 10.1111/clr.12573

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OBJECTIVES Osteoclasts rapidly form on the surface of bone chips at augmentation sites. The underlying molecular mechanism, however, is unclear. Soluble factors released from bone chips in vitro have a robust impact on mesenchymal cell differentiation. Whether these soluble factors change the differentiation of hematopoietic cells into osteoclasts remains unknown. METHODS Osteoclastogenesis, the formation of tartrate-resistant acid phosphatase-positive multinucleated cells, was studied with murine bone marrow cultures exposed to RANKL and M-CSF, and conditioned medium from fresh (BCM) and demineralized bone matrix (DCM). Histochemical staining, gene and protein expression, as well as viability assays were performed. RESULTS This study shows that BCM had no impact on osteoclastogenesis. However, when BCM was heated to 85°C (BCMh), the number of tartrate-resistant acid phosphatase-positive multinucleated cells that developed in the presence of RANKL and M-CSF approximately doubled. In line with the histochemical observations, there was a trend that BCMh increased expression of osteoclast marker genes, in particular the transcription factor c-fos. The expression of c-fos was significantly reduced by the TGF-β receptor I antagonist SB431542. DCM significantly stimulated osteoclastogenesis, independent of thermal processing. CONCLUSIONS These data demonstrate that activated BCM by heat and DBM are able to stimulate osteoclastogenesis in vitro. These in vitro results support the notion that the resorption of autografts may be supported by as yet less defined paracrine mechanisms.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research

UniBE Contributor:

Caballé Serrano, Jordi; Bosshardt, Dieter; Buser, Daniel and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0905-7161

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

18 Feb 2016 09:27

Last Modified:

11 Sep 2017 15:22

Publisher DOI:

10.1111/clr.12573

PubMed ID:

25754222

Uncontrolled Keywords:

TGF-β, allograft, autografts, autologous bone, bone regeneration, bone-conditioned medium, demineralized freeze-dried bone, graft consolidation, osteoclast, paracrine

BORIS DOI:

10.7892/boris.75634

URI:

https://boris.unibe.ch/id/eprint/75634

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