The Rice Transcription Factor WRKY53 Suppresses Herbivore-Induced Defenses by Acting as a Negative Feedback Modulator of Mitogen-Activated Protein Kinase Activity

Hu, Lingfei; Ye, Meng; Li, Ran; Zhang, Tongfang; Zhou, Guoxin; Wang, Qi; Lu, Jing; Lou, Yonggen (2015). The Rice Transcription Factor WRKY53 Suppresses Herbivore-Induced Defenses by Acting as a Negative Feedback Modulator of Mitogen-Activated Protein Kinase Activity. Plant Physiology, 169(4), pp. 2907-2921. American Society of Plant Physiologists 10.1104/pp.15.01090

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The mechanisms by which herbivore-attacked plants activate their defenses are well studied. By contrast, little is known about the regulatory mechanisms that allow them to control their defensive investment and avoid a defensive overshoot. We characterized a rice (Oryza sativa) WRKY gene, OsWRKY53, whose expression is rapidly induced upon wounding and induced in a delayed fashion upon attack by the striped stem borer (SSB) Chilo suppressalis. The transcript levels of OsWRKY53 are independent of endogenous jasmonic acid but positively regulated by the mitogen-activated protein kinases OsMPK3/OsMPK6. OsWRKY53 physically interacts with OsMPK3/OsMPK6 and suppresses their activity in vitro. By consequence, it modulates the expression of defensive, MPK-regulated WRKYs and thereby reduces jasmonic acid, jasmonoyl-isoleucine, and ethylene induction. This phytohormonal reconfiguration is associated with a reduction in trypsin protease inhibitor activity and improved SSB performance. OsWRKY53 is also shown to be a negative regulator of plant growth. Taken together, these results show that OsWRKY53 functions as a negative feedback modulator of MPK3/MPK6 and thereby acts as an early suppressor of induced defenses. OsWRKY53 therefore enables rice plants to control the magnitude of their defensive investment during early signaling.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Hu, Lingfei

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0889

Publisher:

American Society of Plant Physiologists

Funders:

[57] National Natural Science Foundation of China
[58] e Innovation Research Team Program of the National Natural Science Foundation of China
[59] Special Fund for Agroscientific Research in the Public Interest
[60] China Agriculture Research System

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

22 Feb 2016 12:31

Last Modified:

09 Sep 2017 13:06

Publisher DOI:

10.1104/pp.15.01090

PubMed ID:

26453434

BORIS DOI:

10.7892/boris.75726

URI:

https://boris.unibe.ch/id/eprint/75726

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