Comparison of two protocols of periosteal distraction osteogenesis in a rabbit calvaria model

Saulacic, Nikola; Nakahara, Ken; Iizuka, Tateyuki; Haga-Tsujimura, Maiko; Hofstetter, Wilhelm; Scolozzi, Paolo (2016). Comparison of two protocols of periosteal distraction osteogenesis in a rabbit calvaria model. Journal of biomedical materials research. Part B - applied biomaterials, 104(6), pp. 1121-1131. John Wiley & Sons 10.1002/jbm.b.33461

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The regenerative pathways during periosteal distraction osteogenesis may be influenced by the local environment composed by cells, growth factors, nutrition and mechanical load. The aim of the present study was to evaluate the influence of two protocols of periosteal distraction on bone formation. Custom made distraction devices were surgically fixed onto the calvariae of 60 rabbits. After an initial healing period of 7 days, two groups of animals were submitted to distraction rates of 0.25 and 0.5 mm/24 h for 10 days, respectively. Six animals per group were sacrificed 10 (mid-distraction), 17 (end-distraction), 24 (1-week consolidation), 31 (2-week consolidation) and 77 days (2-month consolidation) after surgery. Newly formed bone was assessed by means of micro-CT and histologically. Expression of transcripts encoding tissue-specific genes (BMP-2, RUNX2, ACP5, SPARC, collagen I α1, collagen II α1 and SOX9) was analyzed by quantitative PCR. Two patterns of bone formation were observed, originating from the old bone surface in Group I and from the periosteum in Group II. Bone volume (BV) and bone mineral density (BMD) significantly increased up to the 2-month consolidation period within the groups (p < 0.05). Significantly more bone was observed in Group II compared to Group I at the 2-month consolidation period (p < 0.001). Expression of transcripts encoding osteogenic genes in bone depended on the time-point of observation (p < 0.05). Low level of transcripts reveals an indirect role of periosteum in the osteogenic process. Two protocols of periosteal distraction in the present model resulted in moderate differences in terms of bone formation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Saulacic, Nikola; Nakahara, Ken; Iizuka, Tateyuki; Haga-Tsujimura, Maiko and Hofstetter, Wilhelm

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1552-4973

Publisher:

John Wiley & Sons

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

24 Feb 2016 17:08

Last Modified:

12 Jun 2017 14:43

Publisher DOI:

10.1002/jbm.b.33461

PubMed ID:

26036193

Uncontrolled Keywords:

animal model; bone; distraction osteogenesis; gene expression; histology; micro-CT; periosteum

BORIS DOI:

10.7892/boris.76000

URI:

https://boris.unibe.ch/id/eprint/76000

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