2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli.

Kalbermatter, David; Jeckelmann, Jean-Marc; Chiu, Po-Lin; Ucurum Fotiadis, Zöhre; Walz, Thomas; Fotiadis, Dimitrios José (2015). 2D and 3D crystallization of the wild-type IIC domain of the glucose PTS transporter from Escherichia coli. Journal of structural biology, 191(3), pp. 376-380. Elsevier 10.1016/j.jsb.2015.08.003

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The bacterial phosphoenolpyruvate: sugar phosphotransferase system serves the combined uptake and phosphorylation of carbohydrates. This structurally and functionally complex system is composed of several conserved functional units that, through a cascade of phosphorylated intermediates, catalyze the transfer of the phosphate moiety from phosphoenolpyruvate to the substrate, which is bound to the integral membrane domain IIC. The wild-type glucose-specific IIC domain (wt-IIC(glc)) of Escherichia coli was cloned, overexpressed and purified for biochemical and functional characterization. Size-exclusion chromatography and scintillation-proximity binding assays showed that purified wt-IIC(glc) was homogenous and able to bind glucose. Crystallization was pursued following two different approaches: (i) reconstitution of wt-IIC(glc) into a lipid bilayer by detergent removal through dialysis, which yielded tubular 2D crystals, and (ii) vapor-diffusion crystallization of detergent-solubilized wt-IIC(glc), which yielded rhombohedral 3D crystals. Analysis of the 2D crystals by cryo-electron microscopy and the 3D crystals by X-ray diffraction indicated resolutions of better than 6Å and 4Å, respectively. Furthermore, a complete X-ray diffraction data set could be collected and processed to 3.93Å resolution. These 2D and 3D crystals of wt-IIC(glc) lay the foundation for the determination of the first structure of a bacterial glucose-specific IIC domain.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Kalbermatter, David; Jeckelmann, Jean-Marc; Ucurum Fotiadis, Zöhre and Fotiadis, Dimitrios José

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1047-8477

Publisher:

Elsevier

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

26 Feb 2016 13:24

Last Modified:

26 Feb 2016 13:24

Publisher DOI:

10.1016/j.jsb.2015.08.003

PubMed ID:

26260226

Uncontrolled Keywords:

Cryo-electron microscopy; Membrane protein; Scintillation-proximity assay; Three-dimensional crystal; Transport protein; Two-dimensional crystal; X-ray crystallography

BORIS DOI:

10.7892/boris.76082

URI:

https://boris.unibe.ch/id/eprint/76082

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