High-resolution atomic force microscopy imaging of rhodopsin in rod outer segment disk membranes.

Bosshart, Patrick; Engel, Andreas; Fotiadis, Dimitrios José (2015). High-resolution atomic force microscopy imaging of rhodopsin in rod outer segment disk membranes. Methods in molecular biology, 1271, pp. 189-203. Humana Press 10.1007/978-1-4939-2330-4_13

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Atomic force microscopy (AFM) is a powerful imaging technique that allows recording topographical information of membrane proteins under near-physiological conditions. Remarkable results have been obtained on membrane proteins that were reconstituted into lipid bilayers. High-resolution AFM imaging of native disk membranes from vertebrate rod outer segments has unveiled the higher-order oligomeric state of the G protein-coupled receptor rhodopsin, which is highly expressed in disk membranes. Based on AFM imaging, it has been demonstrated that rhodopsin assembles in rows of dimers and paracrystals and that the rhodopsin dimer is the fundamental building block of higher-order structures.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Bosshart, Patrick and Fotiadis, Dimitrios José

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1064-3745

Publisher:

Humana Press

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

26 Feb 2016 14:10

Last Modified:

16 Sep 2017 17:05

Publisher DOI:

10.1007/978-1-4939-2330-4_13

PubMed ID:

25697525

BORIS DOI:

10.7892/boris.76088

URI:

https://boris.unibe.ch/id/eprint/76088

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