Impact of platelet rich plasma and adipose stem cells on lymphangiogenesis in a murine tail lymphedema model.

Ackermann, Maximilian; Wettstein, Reto; Senaldi, Christopher; Kalbermatten, Daniel F; Konerding, Moritz A; Raffoul, Wassim; Erba, Paolo (2015). Impact of platelet rich plasma and adipose stem cells on lymphangiogenesis in a murine tail lymphedema model. Microvascular research, 102, pp. 78-85. Elsevier 10.1016/j.mvr.2015.09.001

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BACKGROUND Lymphedema is an underdiagnosed pathology which in industrialized countries mainly affects cancer patients that underwent lymph node dissection and/or radiation. Currently no effective therapy is available so that patients' life quality is compromised by swellings of the concerned body region. This unfortunate condition is associated with body imbalance and subsequent osteochondral deformations and impaired function as well as with an increased risk of potentially life threatening soft tissue infections. METHODS The effects of PRP and ASC on angiogenesis (anti-CD31 staining), microcirculation (Laser Doppler Imaging), lymphangiogenesis (anti-LYVE1 staining), microvascular architecture (corrosion casting) and wound healing (digital planimetry) are studied in a murine tail lymphedema model. RESULTS Wounds treated by PRP and ASC healed faster and showed a significantly increased epithelialization mainly from the proximal wound margin. The application of PRP induced a significantly increased lymphangiogenesis while the application of ASC did not induce any significant change in this regard. CONCLUSIONS PRP and ASC affect lymphangiogenesis and lymphedema development and might represent a promising approach to improve regeneration of lymphatic vessels, restore disrupted lymphatic circulation and treat or prevent lymphedema alone or in combination with currently available lymphedema therapies.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ear, Nose and Throat Disorders (ENT)

UniBE Contributor:

Erba, Paolo

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0026-2862

Publisher:

Elsevier

Language:

English

Submitter:

Markus Huth

Date Deposited:

19 Feb 2016 13:45

Last Modified:

19 Feb 2016 13:45

Publisher DOI:

10.1016/j.mvr.2015.09.001

PubMed ID:

26365474

Uncontrolled Keywords:

Adipose stem cells; Angiogenesis; Corrosion casting; Lymphatic regeneration; Lymphoedema; PRP

BORIS DOI:

10.7892/boris.76314

URI:

https://boris.unibe.ch/id/eprint/76314

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