Dual Role of Mesenchymal Stem Cells Allows for Microvascularized Bone Tissue-Like Environments in PEG Hydrogels.

Blache, Ulrich; Metzger, Stéphanie; Vallmajo-Martin, Queralt; Martin, Ivan; Djonov, Valentin; Ehrbar, Martin (2016). Dual Role of Mesenchymal Stem Cells Allows for Microvascularized Bone Tissue-Like Environments in PEG Hydrogels. Advanced healthcare materials, 5(4), pp. 489-498. Wiley 10.1002/adhm.201500795

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In vitro engineered tissues which recapitulate functional and morphological properties of bone marrow and bone tissue will be desirable to study bone regeneration under fully controlled conditions. Among the key players in the initial phase of bone regeneration are mesenchymal stem cells (MSCs) and endothelial cells (ECs) that are in close contact in many tissues. Additionally, the generation of tissue constructs for in vivo transplantations has included the use of ECs since insufficient vascularization is one of the bottlenecks in (bone) tissue engineering. Here, 3D cocultures of human bone marrow derived MSCs (hBM-MSCs) and human umbilical vein endothelial cells (HUVECs) in synthetic biomimetic poly(ethylene glycol) (PEG)-based matrices are directed toward vascularized bone mimicking tissue constructs. In this environment, bone morphogenetic protein-2 (BMP-2) or fibroblast growth factor-2 (FGF-2) promotes the formation of vascular networks. However, while osteogenic differentiation is achieved with BMP-2, the treatment with FGF-2 suppressed osteogenic differentiation. Thus, this study shows that cocultures of hBM-MSCs and HUVECs in biological inert PEG matrices can be directed toward bone and bone marrow-like 3D tissue constructs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

UniBE Contributor:

Djonov, Valentin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2192-2659

Publisher:

Wiley

Language:

English

Submitter:

Ruslan Hlushchuk

Date Deposited:

23 Feb 2016 11:40

Last Modified:

23 Feb 2016 11:40

Publisher DOI:

10.1002/adhm.201500795

PubMed ID:

26693678

Uncontrolled Keywords:

3D tissue model; mesenchymal stem cells; microvascularization; osteogenesis; synthetic hydrogel

BORIS DOI:

10.7892/boris.76429

URI:

https://boris.unibe.ch/id/eprint/76429

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