The presence of iodinated contrast agents amplifies DNA radiation damage in computed tomography

Pathe, Caroline; Eble, Katharina; Schmitz-Beuting, Daniel; Keil, Boris; Kaestner, Bjoern; Voelker, Maximilian; Kleb, Beate; Klose, Klaus J; Heverhagen, Johannes T (2011). The presence of iodinated contrast agents amplifies DNA radiation damage in computed tomography. Contrast media & molecular imaging, 6(6), pp. 507-13. Chichester: Wiley 10.1002/cmmi.453

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The purpose of this study was to determine the influence of iodinated contrast agents on the formation of DNA double-strand breaks in vitro in lymphocytes and to verify these results in patients undergoing diagnostic computed tomography examinations. Blood samples were irradiated in vitro in the presence of iodinated X-ray contrast agent. Controls were irradiated without contrast agent. Fourteen patients were investigated using contrast-enhanced computed tomography (CT), and 14 other patients with unenhanced CT. Blood samples were taken prior to and 5 min and 1, 2 and 24 h after the CT examination. In these blood samples the average number of γH2Ax-foci per lymphocyte was enumerated by fluorescence microscopy. Statistical differences between foci numbers developed in the presence and absence of contrast agent were tested using an independent sample t-test. In vitro foci numbers after irradiation were significantly higher when contrast agent was present during irradiation. In vivo, γH2Ax-foci levels were 58% higher in patients undergoing contrast-enhanced CT compared with those undergoing unenhanced CT. In the presence of iodinated contrast agents DNA, damage is increased and the radiation dose is not the only factor affecting the amount of DNA damage. Individual patient characteristics and biological dosimetry applications, e.g. the analysis of γH2Ax-foci, have to be considered.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology

UniBE Contributor:

Heverhagen, Johannes

ISSN:

1555-4309

Publisher:

Wiley

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:22

Last Modified:

06 Dec 2013 13:28

Publisher DOI:

10.1002/cmmi.453

PubMed ID:

22144029

Web of Science ID:

000297692700011

URI:

https://boris.unibe.ch/id/eprint/7651 (FactScience: 212962)

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