Different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions: Microhardness and polarized light miscroscopy analyses

De Campos, Priscila Hernández; Sanabe, Mariane Emi; Rodrigues, Jonas Almeida; Duarte, Danilo Antonio; Santos, Maria Teresa Botti Rodrigues; Guaré, Renata Oliveira; Duque, Cristiane; Lussi, Adrian; Diniz, Michele Baffi (2015). Different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions: Microhardness and polarized light miscroscopy analyses. Microscopy research and technique, 78(6), pp. 444-451. Wiley-Liss 10.1002/jemt.22493

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The aim of this study was to compare different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions by microhardness and polarized light microscopy analyses. One hundred blocks of bovine enamel were randomly divided into four groups (n = 25) according to the bacterial model for caries induction: (A) Streptococcus mutans, (B) S. mutans and Lactobacillus acidophilus, (C) S. mutans and L. casei, and (D) S. mutans, L. acidophilus, and L. casei. Within each group, the blocks were randomly divided into five subgroups according to the duration of the period of caries induction (4-20 days). The enamel blocks were immersed in cariogenic solution containing the microorganisms, which was changed every 48 h. Groups C and D presented lower surface hardness values (SMH) and higher area of hardness loss (ΔS) after the cariogenic challenge than groups A and B (P < 0.05). As regards lesion depth, under polarized light microscopy, group A presented significantly lower values, and groups C and D the highest values. Group B showed a higher value than group A (P < 0.05). Groups A and B exhibited subsurface caries lesions after all treatment durations, while groups C and D presented erosion-type lesions with surface softening. The model using S. mutans, whether or not it was associated with L. acidophilus, was less aggressive and may be used for the induction of non-cavitated enamel caries-like lesions. The optimal period for inducing caries-like lesions was 8 days.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Lussi, Adrian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1059-910X

Publisher:

Wiley-Liss

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

17 Mar 2016 10:03

Last Modified:

14 Sep 2017 18:32

Publisher DOI:

10.1002/jemt.22493

PubMed ID:

25783414

Uncontrolled Keywords:

bacteria, hardness tests, microscopy, tooth demineralization, tooth enamel

BORIS DOI:

10.7892/boris.77056

URI:

https://boris.unibe.ch/id/eprint/77056

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