First Staphylococcal Cassette Chromosome mec Containing a mecB-Carrying Gene Complex Independent of Transposon Tn6045 in a Macrococcus caseolyticus Isolate from a Canine Infection.

Gomez Sanz, Elena; Schwendener, Sybille; Thomann, Andreas; Gobeli Brawand, Stefanie; Perreten, Vincent (2015). First Staphylococcal Cassette Chromosome mec Containing a mecB-Carrying Gene Complex Independent of Transposon Tn6045 in a Macrococcus caseolyticus Isolate from a Canine Infection. Antimicrobial agents and chemotherapy, 59(8), pp. 4577-4583. American Society for Microbiology 10.1128/AAC.05064-14

[img]
Preview
Text
Antimicrob. Agents Chemother.-2015-Gómez-Sanz-4577-83.pdf - Published Version
Available under License Publisher holds Copyright.

Download (1MB) | Preview

A methicillin-resistant mecB-positive Macrococcus caseolyticus (strain KM45013) was isolated from the nares of a dog with rhinitis. It contained a novel 39-kb transposon-defective complete mecB-carrying staphylococcal cassette chromosome mec element (SCCmecKM45013). SCCmecKM45013 contained 49 coding sequences (CDSs), was integrated at the 3' end of the chromosomal orfX gene, and was delimited at both ends by imperfect direct repeats functioning as integration site sequences (ISSs). SCCmecKM45013 presented two discontinuous regions of homology (SCCmec coverage of 35%) to the chromosomal and transposon Tn6045-associated SCCmec-like element of M. caseolyticus JCSC7096: (i) the mec gene complex (98.8% identity) and (ii) the ccr-carrying segment (91.8% identity). The mec gene complex, located at the right junction of the cassette, also carried the β-lactamase gene blaZm (mecRm-mecIm-mecB-blaZm). SCCmecKM45013 contained two cassette chromosome recombinase genes, ccrAm2 and ccrBm2, which shared 94.3% and 96.6% DNA identity with those of the SCCmec-like element of JCSC7096 but shared less than 52% DNA identity with the staphylococcal ccrAB and ccrC genes. Three distinct extrachromosomal circularized elements (the entire SCCmecKM45013, ΨSCCmecKM45013 lacking the ccr genes, and SCCKM45013 lacking mecB) flanked by one ISS copy, as well as the chromosomal regions remaining after excision, were detected. An unconventional circularized structure carrying the mecB gene complex was associated with two extensive direct repeat regions, which enclosed two open reading frames (ORFs) (ORF46 and ORF51) flanking the chromosomal mecB-carrying gene complex. This study revealed M. caseolyticus as a potential disease-associated bacterium in dogs and also unveiled an SCCmec element carrying mecB not associated with Tn6045 in the genus Macrococcus.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology > Molecular Bacterial Epidemiology and Infectiology
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Gomez Sanz, Elena; Schwendener, Sybille; Thomann, Andreas; Gobeli, Stefanie and Perreten, Vincent

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0066-4804

Publisher:

American Society for Microbiology

Funders:

[UNSPECIFIED] Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), no. 21/2012
[UNSPECIFIED] Institute of Veterinary Bacteriology, University of Bern, no. 35-539

Language:

English

Submitter:

Vincent Perreten

Date Deposited:

02 Mar 2016 12:25

Last Modified:

08 Feb 2017 14:29

Publisher DOI:

10.1128/AAC.05064-14

PubMed ID:

25987634

BORIS DOI:

10.7892/boris.77116

URI:

https://boris.unibe.ch/id/eprint/77116

Actions (login required)

Edit item Edit item
Provide Feedback