Endothelial- and Platelet-Derived Microparticles Are Generated During Liver Resection in Humans.

Banz Wälti, Yara; Item, Gian-Marco; Vogt, Andreas; Rieben, Robert; Candinas, Daniel; Beldi, Guido (2015). Endothelial- and Platelet-Derived Microparticles Are Generated During Liver Resection in Humans. Journal of investigative surgery, 29(1), pp. 20-31. Taylor & Francis 10.3109/08941939.2015.1047540

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BACKGROUND

Cell-derived plasma microparticles (<1.5 μm) originating from various cell types have the potential to regulate thrombogenesis and inflammatory responses. The aim of this study was to test the hypothesis that microparticles generated during hepatic surgery co-regulate postoperative procoagulant and proinflammatory events.

METHODS

In 30 patients undergoing liver resection, plasma microparticles were isolated, quantitated, and characterized as endothelial (CD31+, CD41-), platelet (CD41+), or leukocyte (CD11b+) origin by flow cytometry and their procoagulant and proinflammatory activity was measured by immunoassays.

RESULTS

During liver resection, the total numbers of microparticles increased with significantly more Annexin V-positive, endothelial and platelet-derived microparticles following extended hepatectomy compared to standard and minor liver resections. After liver resection, microparticle tissue factor and procoagulant activity increased along with overall coagulation as assessed by thrombelastography. Levels of leukocyte-derived microparticles specifically increased in patients with systemic inflammation as assessed by C-reactive protein but are independent of the extent of liver resection.

CONCLUSIONS

Endothelial and platelet-derived microparticles are specifically elevated during liver resection, accompanied by increased procoagulant activity. Leukocyte-derived microparticles are a potential marker for systemic inflammation. Plasma microparticles may represent a specific response to surgical stress and may be an important mediator of postoperative coagulation and inflammation.

Item Type:

 Journal Article (Original Article)

Division/Institute:

 04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Viszeralchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Herz und Gefässe
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Anästhesiologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Anästhesiologie
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

UniBE Contributor:

 Banz Wälti, Yara Sarah, Vogt, Andreas, Rieben, Robert, Candinas, Daniel, Beldi, Guido Jakob Friedrich

Subjects:

 600 Technology > 610 Medicine & health

ISSN:

 0894-1939

Publisher:

 Taylor & Francis

Language:

 English

Submitter:

 Lilian Karin Smith-Wirth

Date Deposited:

 23 Mar 2016 08:37

Last Modified:

 02 Mar 2023 23:27

Publisher DOI:

 10.3109/08941939.2015.1047540

PubMed ID:

 26375814

Uncontrolled Keywords:

 Microparticles; endothelium; liver resection; patients; platelets

BORIS DOI:

 10.7892/boris.77484

URI:

 https://boris.unibe.ch/id/eprint/77484

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