Rescue of neonatal cardiac dysfunction in mice by administration of cardiac progenitor cells in utero

Liu, Xiaoli; Hall, Sean; Wang, Zhihong; Huang, He; Ghanta, Sailaja; Di Sante, Moises; Leri, Annarosa; Anversa, Piero; Perrella, Mark A (2015). Rescue of neonatal cardiac dysfunction in mice by administration of cardiac progenitor cells in utero. Nature communications, 6, p. 8825. Nature Publishing Group 10.1038/ncomms9825

[img]
Preview
Text
ncomms9825.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (3MB) | Preview

Striated preferentially expressed gene (Speg) is a member of the myosin light chain kinase family. We previously showed that disruption of the Speg gene locus in mice leads to a dilated cardiomyopathy with immature-appearing cardiomyocytes. Here we show that cardiomyopathy of Speg(-/-) mice arises as a consequence of defects in cardiac progenitor cell (CPC) function, and that neonatal cardiac dysfunction can be rescued by in utero injections of wild-type CPCs into Speg(-/-) foetal hearts. CPCs harvested from Speg(-/-) mice display defects in clone formation, growth and differentiation into cardiomyocytes in vitro, which are associated with cardiac dysfunction in vivo. In utero administration of wild-type CPCs into the hearts of Speg(-/-) mice results in CPC engraftment, differentiation and myocardial maturation, which rescues Speg(-/-) mice from neonatal heart failure and increases the number of live births by fivefold. We propose that in utero administration of CPCs may have future implications for treatment of neonatal heart diseases.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Thoracic Surgery

UniBE Contributor:

Hall, Sean

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2041-1723

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Thomas Michael Marti

Date Deposited:

04 Apr 2016 13:27

Last Modified:

08 Apr 2016 05:48

Publisher DOI:

10.1038/ncomms9825

PubMed ID:

26593099

BORIS DOI:

10.7892/boris.77786

URI:

https://boris.unibe.ch/id/eprint/77786

Actions (login required)

Edit item Edit item
Provide Feedback