Vaccination of cattle with the N terminus of LppQ of Mycoplasma mycoides subsp. mycoides results in type III immune complex disease upon experimental infection.

Mulongo, Musa; Frey, Joachim; Smith, Ken; Schnier, Christian; Wesonga, Hezron; Naessens, Jan; McKeever, Declan (2015). Vaccination of cattle with the N terminus of LppQ of Mycoplasma mycoides subsp. mycoides results in type III immune complex disease upon experimental infection. Infection and immunity, 83(5), pp. 1992-2000. American Society for Microbiology 10.1128/IAI.00003-15

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Contagious bovine pleuropneumonia (CBPP) is a serious respiratory disease of cattle caused by Mycoplasma mycoides subsp. mycoides. Current vaccines against CBPP induce short-lived immunity and can cause severe postvaccine reactions. Previous studies have identified the N terminus of the transmembrane lipoprotein Q (LppQ-N') of M. mycoides subsp. mycoides as the major antigen and a possible virulence factor. We therefore immunized cattle with purified recombinant LppQ-N' formulated in Freund's adjuvant and challenged them with M. mycoides subsp. mycoides. Vaccinated animals showed a strong seroconversion to LppQ, but they exhibited significantly enhanced postchallenge glomerulonephritis compared to the placebo group (P = 0.021). Glomerulonephritis was characterized by features that suggested the development of antigen-antibody immune complexes. Clinical signs and gross pathological scores did not significantly differ between vaccinated and placebo groups. These findings reveal for the first time the pathogenesis of enhanced disease as a result of antibodies against LppQ during challenge and also argue against inclusion of LppQ-N' in a future subunit vaccine for CBPP.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Frey, Joachim

Subjects:

600 Technology > 630 Agriculture

ISSN:

0019-9567

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Barbara Gautschi-Steffen

Date Deposited:

29 Mar 2016 15:25

Last Modified:

29 Mar 2016 15:25

Publisher DOI:

10.1128/IAI.00003-15

PubMed ID:

25733516

BORIS DOI:

10.7892/boris.77853

URI:

https://boris.unibe.ch/id/eprint/77853

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