Panels of cytokines and other secretory proteins as potential biomarkers of ovarian endometriosis.

Kocbek, Vida; Vouk, Katja; Bersinger, Nick A.; Mueller, Michael; Lanišnik Rižner, Tea (2015). Panels of cytokines and other secretory proteins as potential biomarkers of ovarian endometriosis. The Journal of molecular diagnostics, 17(3), pp. 325-334. Elsevier 10.1016/j.jmoldx.2015.01.006

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Endometriosis is a gynecologic disease that is characterized by nonspecific symptoms and invasive diagnostics. To date, there is no adequate noninvasive method for the diagnosis of endometriosis. Although more than 100 potential biomarkers have been investigated in blood and/or peritoneal fluid, none of these has proven useful in clinical practice. The aim to find a suitable panel of biomarkers that would allow noninvasive diagnosis thus remains of interest. We evaluated the concentrations of 16 cytokines and other secretory proteins in serum and peritoneal fluid of 58 women with ovarian endometriosis (cases) and 40 healthy women undergoing sterilization or patients with benign ovarian cysts (controls) using multiplexed double fluorescence-based immunometric assay platform and enzyme-linked immunosorbent assay. Significantly higher concentrations of glycodelin-A were shown in serum, and significantly higher levels of glycodelin-A, IL-6, and IL-8, and lower levels of leptin were measured in the peritoneal fluid of cases versus controls. In serum, the best performance was shown by models that included the ratio of leptin/glycodelin-A and the ratio of ficolin 2/glycodelin-A, whereas in the peritoneal fluid the best models included the ratio of biglycan/leptin, regulated on activation normal T-cell expressed and secreted/IL-6 and ficolin-2/glycodelin-A, and IL-8 per milligram of total protein, all in combination with age. The models using serum and peritoneal fluid distinguished between ovarian endometriosis patients and controls regardless of the menstrual cycle phase with relatively high sensitivity (72.5% to 84.2%), specificity (78.4% to 91.2%), and area under the curve (0.85 to 0.90).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Gynaecology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Endometriose und gynäkologische Onkologie

UniBE Contributor:

Kocbek, Vida; Bersinger, Nick A. and Mueller, Michael

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1943-7811

Publisher:

Elsevier

Language:

English

Submitter:

Monika Zehr

Date Deposited:

04 Mar 2016 10:11

Last Modified:

04 Mar 2016 10:11

Publisher DOI:

10.1016/j.jmoldx.2015.01.006

PubMed ID:

25797583

BORIS DOI:

10.7892/boris.77855

URI:

https://boris.unibe.ch/id/eprint/77855

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