Effect of Enamel Matrix Derivative Liquid on Osteoblast and Periodontal Ligament Cell Proliferation and Differentiation.

Miron, Richard John; Chandad, Fatiha; Buser, Daniel; Sculean, Anton; Cochran, David L; Zhang, Yufeng (2016). Effect of Enamel Matrix Derivative Liquid on Osteoblast and Periodontal Ligament Cell Proliferation and Differentiation. Journal of periodontology, 87(1), pp. 91-99. American Academy of Periodontology 10.1902/jop.2015.150389

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BACKGROUND Enamel matrix derivatives (EMDs) have been used clinically for more than a decade for the regeneration of periodontal tissues. The aim of the present study is to analyze the effect on cell growth of EMDs in a gel carrier in comparison to EMDs in a liquid carrier. EMDs in a liquid carrier have been shown to adsorb better to bone graft materials. METHODS Primary human osteoblasts and periodontal ligament (PDL) cells were exposed to EMDs in both gel and liquid carriers and compared for their ability to induce cell proliferation and differentiation. Alizarin red staining and real-time polymerase chain reaction for expression of genes encoding collagen 1, osteocalcin, and runt-related transcription factor 2, as well as bone morphogenetic protein 2 (BMP2), transforming growth factor (TGF)-β1, and interleukin (IL)-1β, were assessed. RESULTS EMDs in both carriers significantly increased cell proliferation of both osteoblasts and PDL cells in a similar manner. Both formulations also significantly upregulated the expression of genes encoding BMP2 and TGF-β1 as well as decreased the expression of IL-1β. EMDs in the liquid carrier further retained similar differentiation potential of both osteoblasts and PDL cells by demonstrating increased collagen and osteocalcin gene expression and significantly higher alizarin red staining. CONCLUSIONS The results from the present study indicate that the new formulation of EMDs in a liquid carrier is equally as potent as EMDs in a gel carrier in inducing osteoblast and PDL activity. Future study combining EMDs in a liquid carrier with bone grafting materials is required to further evaluate its potential for combination therapies.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology

UniBE Contributor:

Miron, Richard John; Buser, Daniel and Sculean, Anton

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-3492

Publisher:

American Academy of Periodontology

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

14 Apr 2016 12:30

Last Modified:

14 Apr 2016 12:30

Publisher DOI:

10.1902/jop.2015.150389

PubMed ID:

26334247

Uncontrolled Keywords:

Bone Transplantation, enamel matrix Proteins, gene Expression, regeneration, periodontal guided tissue

BORIS DOI:

10.7892/boris.78446

URI:

https://boris.unibe.ch/id/eprint/78446

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