RASSF7 expression and its regulatory roles on apoptosis in human intervertebral disc degeneration

Liu, Zhi-Heng; Huo, Jun-Li; Wu, Zhi-Gang; Sun, Zhen; Bai, Feng; Samartzis, Dino; Gantenbein, Benjamin; Fan, Shao-Di; Wang, Hai-Qiang (2015). RASSF7 expression and its regulatory roles on apoptosis in human intervertebral disc degeneration. International journal of clinical and experimental pathology, 8(12), pp. 16097-16103. e-Century Publishing

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Apoptosis plays an important role in intervertebral disc degeneration (IDD). Overwhelming evidence indicates that RASSF7 is essential for cell growth and apoptosis. Recently, it has been noted that the JNK signaling can be negatively regulated by suppressing phosphorylated-MKK7 activation during pro-apoptosis. We aimed to investigate the RASSF7 expression level in human degenerative nucleus pulposus (NP) cells and non-degenerative NP cells and the link between RASSF7-JNK with NP cells apoptosis. We harvested NP tissues from 20 IDD patients as disease group and 8 cadaveric donors as normal controls. We detected RASSF7 expression by Real-time-PCR and western blotting. Consequently, we found that the expression of RASSF7 was higher in non-degenerative group than in degenerative group (P<0.05). Overexpression of RASSF7 in degenerative NP cells led to decreased apoptosis rate than that in scramble group (P<0.05). Collectively, our findings suggest that RASSF7 plays an important role in human IDD and RASSF7 might be potentially developed as a curative agent.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB

UniBE Contributor:

Gantenbein, Benjamin

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1936-2625

Publisher:

e-Century Publishing

Language:

English

Submitter:

Benjamin Gantenbein

Date Deposited:

06 Apr 2016 13:26

Last Modified:

06 Apr 2016 13:26

PubMed ID:

26884887

Uncontrolled Keywords:

Intervertebral disc degeneration; JNK; RASSF7; apoptosis; nucleus pulposus

BORIS DOI:

10.7892/boris.78898

URI:

https://boris.unibe.ch/id/eprint/78898

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