Inhibition of glutathione synthesis reduces chilling tolerance in maize

Kocsy, Gabor; von Ballmoos, Peter; Suter, Marianne; Rüegsegger, Adrian; Galli, Ulrich; Szalai, Gabriella; Galiba, Gabor; Brunold, Christian (2000). Inhibition of glutathione synthesis reduces chilling tolerance in maize. Planta, 211(4), pp. 528-536. Springer 10.1007/s004250000308

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The role of glutathione (GSH) in protecting plants from chilling injury was analyzed in seedlings of a chilling-tolerant maize (Zea mays L.) genotype using buthionine sulfoximine (BSO), a specific inhibitor of gamma-glutamylcysteine (gamma EC) synthetase, the first enzyme of GSH synthesis. At 25 degrees C, 1 mM BSO significantly increased cysteine and reduced GSH content and GSH reductase (GR: EC 1.6.4.2) activity, but interestingly affected neither fresh weight nor dry weight nor relative injury. Application of BSO up to 1 mM during chilling at 5 degrees C reduced the fresh and dry weights of shoots and roots and increased relative injury from 10 to almost 40%. Buthionine sulfoximine also induced a decrease in GR activity of 90 and 40% in roots and shoots, respectively. Addition of GSH or gamma EC together with BSO to the nutrient solution protected the seedlings from the BSO effect by increasing the levels of GSH and GR activity in roots and shoots. During chilling, the level of abscisic acid increased both in controls and BSO-treated seedlings and decreased after chilling in roots and shoots of the controls and in the roots of BSO-treated seedlings, but increased in their shoots. Taken together, our results show that BSO did not reduce chilling tolerance of the maize genotype analyzed by inhibiting abscisic acid accumulation but by establishing a low level of GSH. which also induced a decrease in GR activity.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

von Ballmoos, Peter; Suter, Marianne and Brunold, Christian

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0935

Publisher:

Springer

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

11 Mar 2016 10:09

Last Modified:

14 Nov 2017 16:24

Publisher DOI:

10.1007/s004250000308

Uncontrolled Keywords:

Abscisic acid, Buthionine sulfoximine, Cysteine, Glutathione, Glutathione reductase, Zea

BORIS DOI:

10.7892/boris.78912

URI:

https://boris.unibe.ch/id/eprint/78912

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