Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration.

González-Rosa, Juan Manuel; Peralta, Marina; Mercader Huber, Nadia (2012). Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration. Developmental biology, 370(2), pp. 173-186. Elsevier 10.1016/j.ydbio.2012.07.007

[img] Text
1-s2.0-S0012160612003788-main.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (4MB) | Request a copy

Myocardial infarction (MI) leads to a severe loss of cardiomyocytes, which in mammals are replaced by scar tissue. Epicardial derived cells (EPDCs) have been reported to differentiate into cardiomyocytes during development, and proposed to have cardiomyogenic potential in the adult heart. However, mouse MI models reveal little if any contribution of EPDCs to myocardium. In contrast to adult mammals, teleosts possess a high myocardial regenerative capacity. To test if this advantage relates to the properties of their epicardium, we studied the fate of EPDCs in cryoinjured zebrafish hearts. To avoid the limitations of genetic labelling, which might trace only a subpopulation of EPDCs, we used cell transplantation to track all EPDCs during regeneration. EPDCs migrated to the injured myocardium, where they differentiated into myofibroblasts and perivascular fibroblasts. However, we did not detect any differentiation of EPDCs nor any other non-cardiomyocyte population into cardiomyocytes, even in a context of impaired cardiomyocyte proliferation. Our results support a model in which the epicardium promotes myocardial regeneration by forming a cellular scaffold, and suggests that it might induce cardiomyocyte proliferation and contribute to neoangiogenesis in a paracrine manner.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Mercader Huber, Nadia

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0012-1606

Publisher:

Elsevier

Language:

English

Submitter:

Nadia Isabel Mercader Huber

Date Deposited:

11 May 2016 08:37

Last Modified:

11 May 2016 08:37

Publisher DOI:

10.1016/j.ydbio.2012.07.007

PubMed ID:

22877945

BORIS DOI:

10.7892/boris.79619

URI:

https://boris.unibe.ch/id/eprint/79619

Actions (login required)

Edit item Edit item
Provide Feedback