Cryoinjury as a myocardial infarction model for the study of cardiac regeneration in the zebrafish.

González-Rosa, Juan Manuel; Mercader Huber, Nadia (2012). Cryoinjury as a myocardial infarction model for the study of cardiac regeneration in the zebrafish. Nature protocols, 7(4), pp. 782-788. Nature Publishing Group 10.1038/nprot.2012.025

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The zebrafish heart has the capacity to regenerate after ventricular resection. Although this regeneration model has proved useful for the elucidation of certain regeneration mechanisms, it is based on the removal of heart tissue rather than on tissue damage. We recently characterized the cellular response and regenerative capacity of the zebrafish heart after cryoinjury (CI), an alternative procedure that more closely models the pathophysiological process undergone by the human heart after myocardial infarction (MI). After anesthesia, localized CI with a liquid nitrogen-cooled copper probe induced damage in 25% of the ventricle, in a procedure requiring <5 min. Here we present a detailed description of the technique, which provides a valuable system for the study of the mechanisms of heart regeneration and scar removal after MI in a versatile vertebrate model.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Mercader Huber, Nadia

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1754-2189

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Nadia Isabel Mercader Huber

Date Deposited:

11 May 2016 08:56

Last Modified:

11 May 2016 08:56

Publisher DOI:

10.1038/nprot.2012.025

PubMed ID:

22461067

BORIS DOI:

10.7892/boris.79621

URI:

https://boris.unibe.ch/id/eprint/79621

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