The Osr1 and Osr2 genes act in the pronephric anlage downstream of retinoic acid signaling and upstream of Wnt2b to maintain pectoral fin development.

Neto, Ana; Mercader Huber, Nadia; Gómez-Skarmeta, José Luis (2012). The Osr1 and Osr2 genes act in the pronephric anlage downstream of retinoic acid signaling and upstream of Wnt2b to maintain pectoral fin development. Development, 139(2), pp. 301-311. Company of Biologists Limited 10.1242/dev.074856

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Vertebrate odd-skipped related genes (Osr) have an essential function during the formation of the intermediate mesoderm (IM) and the kidney structures derived from it. Here, we show that these genes are also crucial for limb bud formation in the adjacent lateral plate mesoderm (LPM). Reduction of zebrafish Osr function impairs fin development by the failure of tbx5a maintenance in the developing pectoral fin bud. Osr morphant embryos show reduced wnt2b expression, and increasing Wnt signaling in Osr morphant embryos partially rescues tbx5a expression. Thus, Osr genes control limb bud development in a non-cell-autonomous manner, probably through the activation of Wnt2b. Finally, we demonstrate that Osr genes are downstream targets of retinoic acid (RA) signaling. Therefore, Osr genes act as a relay within the genetic cascade of fin bud formation: by controlling the expression of the signaling molecule Wnt2ba in the IM they play an essential function transmitting the RA signaling originated in the somites to the LPM.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Mercader Huber, Nadia

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0950-1991

Publisher:

Company of Biologists Limited

Language:

English

Submitter:

Nadia Isabel Mercader Huber

Date Deposited:

11 May 2016 09:06

Last Modified:

11 May 2016 09:06

Publisher DOI:

10.1242/dev.074856

PubMed ID:

22129829

BORIS DOI:

10.7892/boris.79622

URI:

https://boris.unibe.ch/id/eprint/79622

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