Proximodistal identity during vertebrate limb regeneration is regulated by Meis homeodomain proteins

Mercader Huber, Nadia; Tanaka, Elly M; Torres, Miguel (2005). Proximodistal identity during vertebrate limb regeneration is regulated by Meis homeodomain proteins. Development, 132(18), pp. 4131-4142. Company of Biologists Limited 10.1242/dev.01976

[img]
Preview
Text
4131.full.pdf - Published Version
Available under License Publisher holds Copyright.

Download (1MB) | Preview

The mechanisms by which cells obtain instructions to precisely re-create the missing parts of an organ remain an unresolved question in regenerative biology. Urodele limb regeneration is a powerful model in which to study these mechanisms. Following limb amputation, blastema cells interpret the proximal-most positional identity in the stump to reproduce missing parts faithfully. Classical experiments showed the ability of retinoic acid (RA) to proximalize blastema positional values. Meis homeobox genes are involved in RA-dependent specification of proximal cell identity during limb development. To understand the molecular basis for specifying proximal positional identities during regeneration, we isolated the axolotl Meis homeobox family. Axolotl Meis genes are RA-regulated during both regeneration and embryonic limb development. During limb regeneration, Meis overexpression relocates distal blastema cells to more proximal locations, whereas Meis knockdown inhibits RA proximalization of limb blastemas. Meis genes are thus crucial targets of RA proximalizing activity on blastema cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Faculty Institutions > Teaching Staff, Faculty of Medicine

UniBE Contributor:

Mercader Huber, Nadia

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0950-1991

Publisher:

Company of Biologists Limited

Language:

English

Submitter:

Nadia Isabel Mercader Huber

Date Deposited:

30 Jun 2016 16:34

Last Modified:

04 Jul 2016 22:13

Publisher DOI:

10.1242/dev.01976

PubMed ID:

16107473

BORIS DOI:

10.7892/boris.79627

URI:

https://boris.unibe.ch/id/eprint/79627

Actions (login required)

Edit item Edit item
Provide Feedback