Connolly, Mona; Fernandez-Cruz, Maria-Luisa; Quesada-Garcia, Alba; Alte, Luis; Segner, Helmut; Navas, Jose M (2015). Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes. International journal of environmental research and public health, 12(5), pp. 5386-5405. MDPI 10.3390/ijerph120505386
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Among all classes of nanomaterials, silver nanoparticles (AgNPs) have potentially an important ecotoxicological impact, especially in freshwater environments. Fish are particularly susceptible to the toxic effects of silver ions and, with knowledge gaps regarding the contribution of dissolution and unique particle effects to AgNP toxicity, they represent a group of vulnerable organisms. Using cell lines (RTL-W1, RTH-149, RTG-2) and primary hepatocytes of rainbow trout (Oncorhynchus mykiss) as in vitro test systems, we assessed the cytotoxicity of the representative AgNP, NM-300K, and AgNO3 as an Ag+ ion source. Lack of AgNP interference with the cytotoxicity assays (AlamarBlue, CFDA-AM, NRU assay) and their simultaneous application point to the compatibility and usefulness of such a battery of assays. The RTH-149 and RTL-W1 liver cell lines exhibited similar sensitivity as primary hepatocytes towards AgNP toxicity. Leibovitz's L-15 culture medium composition (high amino acid content) had an important influence on the behaviour and toxicity of AgNPs towards the RTL-W1 cell line. The obtained results demonstrate that, with careful consideration, such an in vitro approach can provide valuable toxicological data to be used in an integrated testing strategy for NM-300K risk assessment.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute for Fish and Wildlife Health (FIWI) |
UniBE Contributor: |
Segner, Helmut |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
1661-7827 |
Publisher: |
MDPI |
Language: |
English |
Submitter: |
Lucia Gugger-Raaflaub |
Date Deposited: |
19 Apr 2016 15:28 |
Last Modified: |
07 Aug 2024 15:45 |
Publisher DOI: |
10.3390/ijerph120505386 |
PubMed ID: |
26006119 |
Uncontrolled Keywords: |
AgNPs; cytotoxicity; in vitro cell lines; primary hepatocyte; rainbow trout (Oncorhynchus mykiss) |
BORIS DOI: |
10.7892/boris.79796 |
URI: |
https://boris.unibe.ch/id/eprint/79796 |
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