Determination of Metabolic Stability Using Cryopreserved Hepatocytes from Rainbow Trout (Oncorhynchus mykiss).

Fay, Kellie A; Nabb, Diane L; Mingoia, Robert T; Bischof, Ina Bettina Petra; Nichols, John W; Segner, Helmut; Johanning, Karla; Han, Xing (2015). Determination of Metabolic Stability Using Cryopreserved Hepatocytes from Rainbow Trout (Oncorhynchus mykiss). Current protocols in toxicology, 65(4.42), pp. 1-29. Wiley 10.1002/0471140856.tx0442s65

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Trout provide a relatively easy source of hepatocytes that can be cryopreserved and used for a range of applications including toxicity testing and determination of intrinsic clearance. Standard protocols for isolating, cryopreserving, and thawing rainbow trout hepatocytes are described, along with procedures for using fresh or cryopreserved hepatocytes to assess metabolic stability of xenobiotics in fish by means of a substrate depletion approach. Variations on these methods, troubleshooting tips, and directions for use of extrapolation factors to express results in terms of in vivo intrinsic clearance are included. These protocols have been developed for rainbow trout, but can be adapted to other fish species with appropriate considerations.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Bischof, Ina Bettina Petra and Segner, Helmut

Subjects:

600 Technology > 630 Agriculture

ISSN:

1934-9262

Publisher:

Wiley

Language:

English

Submitter:

Lucia Gugger-Raaflaub

Date Deposited:

20 Apr 2016 07:43

Last Modified:

20 Apr 2016 07:43

Publisher DOI:

10.1002/0471140856.tx0442s65

PubMed ID:

26250400

Uncontrolled Keywords:

biotransformation; fish; hepatocytes; in vitro assay; metabolic stability; rainbow trout; substrate depletion

URI:

https://boris.unibe.ch/id/eprint/80172

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